Survivin在星形细胞瘤中的表达及与血管内皮生长因子和微血管密度的关系

目的　观察Survivin在星形细胞瘤中的表达 ,通过分析其与VEGF和微血管密度的相关性 ,探讨其与肿瘤血管形成的关系。方法　星形细胞瘤手术标本 35例 ,免疫组化方法检测肿瘤组织中的Survivin ,用CD34单抗显示微血管 ,用血管计数 (MVD)定量肿瘤微血管形成。RT -PCR检测肿瘤组织中VEGFmRNA的表达。结果　 1Survivin、VEGFmRNA和MVD在星形细胞瘤中的表达与肿瘤的病理分级呈正相关 (P <0 .0 1)。 2在Survivin阳性标本中VEGFmRNA和MVD的表达均高于Survivin阴性的标本 ,差异有显著性 (P <0 .0 1P <0 .0 5 )。 3Survivin的表达强度与VEGFmRNA的表达行线性回归分析 ,呈显著正相关 ,R =0 .814 ,P <0 .0 1。结论　Survivin的表达随星形细胞瘤的恶性程度升高而增高 ,说明它是肿瘤恶性的特征。Survivin的表达与VEGF和MVD的表达呈正相关 ,提示Survivin可能参与了VEGF相关的肿瘤血管形成

Expression of Survivin in Astrocytoma and its Relation to Vascular Endothelial Growth Factor and Microvessel Density

Purpose To observe the expression of Survivin in astrocytoma of various pathological grades and explore its relation to tumor vascularization. Methods Thirty five astrocytoma samples were obtained from the patients who underwent neurological surgery.Survivin was stained by way of immunohistochemistry and the microvessel was immunostained with anti human CD34 mono antibody.MVD was measured to show tumor vascularization as well as IOD of Survivin positive cells being checked.Semi quantitative RT-PCR was also applied to detect VEGF mRNA expression in astrocytoma. Results The expression of Survivin,VEGF mRNA and MDV were significant positive correlated with the pathological grades of astrocytoma ( P <0.01).Expression of VEGF mRNA and MVD in the Survivin positive group were considerably higher than that of the Survivin negative group ( P < 0.01 and <0.05 respectively).In the Survivin positive group,the Sruvivin expression is significantly positive correlated with VEGF mRNA by the linear regression analysis (R=0.814, P <0.01). Conclusions Survivin tended to express in high grade ascrocytoma,which suggested that it could be a mark of malignant tumor.And that,the expression of survivin went up with VEGF and MVD led to the possibility that Survivin may take park in the VEGF related tumor angiogenesis.