Interleukin-4 downregulates interleukin-6 production in human peripheral blood mononuclear cells |
| |
Authors: | J D Lee S G Swisher E H Minehart W H McBride J S Economou |
| |
Affiliation: | Department of Surgery, UCLA School of Medicine 90024. |
| |
Abstract: | We report that recombinant human interleukin-4 (IL-4) downregulates interleukin-6 (IL-6) production by human peripheral blood mononuclear cells (PBMC). PBMC were preincubated for up to 24 hr in the presence of IL-4 (100 U/ml) and then activated with lipopolysaccharide B Escherichia coli 026:B6 (LPS, 10 micrograms/ml), recombinant human tumor necrosis factor-alpha (TNF-alpha, 200 U/ml), or Concanavalin A (Con A, 10 micrograms/ml). Although all these signals induced IL-6 production, IL-4-treated cells produced significantly reduced levels of IL-6 protein. This effect was dose and time dependent. We conclude that IL-4 is a potent downregulatory modulator of IL-6 expression in human PBMC. |
| |
Keywords: | |
|
|