| 福建口岸输入登革I型病毒E基因序列分析 |
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| 引用本文： | 张建明,;黄野能,;高博,;杨坤宇,;赵书艺,;王宇平.福建口岸输入登革I型病毒E基因序列分析[J].中国国境卫生检疫杂志,2014(4):226-229. |
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| 作者姓名： | 张建明 ;黄野能 ;高博 ;杨坤宇 ;赵书艺 ;王宇平 |
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| 作者单位： | [1]福建国际旅行卫生保健中心,福建福州350001; [2]厦门国际旅行卫生保健中心;,福建福州350001; [3]福建医科大学,福建福州350001; |
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| 基金项目： | 福建省科技计划重点项目（2013Y0002）;国家质检总局科技项目（2013IK221）;福建省自然基金项目（2013J01353） |
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| 摘 要： | 目的研究分析福建口岸输入登革I型病毒E基因序列,为登革热诊断的分子溯源提供参考。方法收集2012—2013年福建口岸截获的7例入境Ⅰ型登革热病例资料及血清标本,用C6/36细胞培养分离病毒,提取病毒RNA,用RT-nested-PCR扩增包含E基因全长的核酸片段,扩增产物经纯化、测序,用生物学软件进行DNA序列比对分析。结果用C6/36细胞从7例登革热病例血清中成功分离出7株登革病毒株,经RT-nested-PCR证实均为DENV-Ⅰ型,构建E基因全长进化树分析发现,分离株与新加坡、越南和菲律宾等地的病毒株同源性最高,与病例入境时进行的流行病学调查资料完全吻合。结论从分子水平证明登革热病例血清中分离到的毒株为DENV-Ⅰ型病毒,结合流行病学调查资料,证实均为输入性感染病例,最有可能来源于东南亚一带。
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| 关 键 词： | 登革病毒 E基因 序列分析 系统进化树 |
Study on E gene sequence of dengue virus type I at Fujian port |
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| Institution: | ZHANG Jian-ming, HUANG Ye-neng, GAO Bo, YANG Kun-yu, ZHAO Shu-yi, WANG Yu-ping (Fujian International Travel Healthcare Center, Fuzhou, Fujian 350001, China) |
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| Abstract: | Objective To study E gene sequence of dengue virus type I at Fujian port, and to lay a foundation for molecular diagnosis of dengue fever. Methods Epidemiological data and serum samples were collected and viruses were isolated in C6/36 cells. The viral RNA was extracted, and amplified to DNA fragment containing the full- length E gene by RT-nested-PCR. The amplified product was purified, sequenced and analyzed with biological software. Results Seven strains of dengue virus isolates from patients at Fujian port were shown to be DENV-I. The full length E gene （1485 bp） was amplified successfully. The phylogenetic tree showed more homology with Singapore, Vietnam and Philippines virus strains, consistent with the source of entry patients. Conelusion A total of 7 cases were imported dengue fever cases. Southeast Asia was the most likely source. |
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| Keywords: | Dengue virus E gene Sequence analysis Phylogenetic tree |
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