cyclin E和p27kip1蛋白在胆囊癌组织中的表达及其意义

目的　探讨cyclinE和 p2 7kip1蛋白在胆囊癌组织中的表达及其意义。方法　采用免疫组化SP法检测 4 1例胆囊癌和 15例慢性胆囊炎组织中cyclinE及p2 7kip1蛋白的表达 ,并分析两者的表达与胆囊癌临床病理特征的关系。结果　cyclinE在胆囊癌组织中的表达阳性率为 6 1.0 % (2 5 / 4 1) ,显著高于慢性胆囊炎的 2 0 .0 % (3/15 ) ,P<0 .0 5 ;胆囊癌组织中p2 7kip1蛋白的表达阳性率为 5 3.7% ,低于慢性胆囊炎的 10 0 % (P<0 .0 5 )。cyclinE的表达与胆囊癌TNM分期呈正相关 (r =0 .314 ,P<0 .0 5 ) ,p2 7kip1蛋白的表达则随胆囊癌TNM分期进展、组织学分化程度的降低而逐渐下降 (P<0 .0 5 ) ,cyclinE与 p2 7kip1蛋白表达之间呈负相关 (r =- 0 .342 ,P<0 .0 5 )。结论　cyclinE蛋白高表达和 p2 7kip1蛋白表达下降导致细胞周期的调控异常 ,可能参与了胆囊癌的发生、发展过程。

Expression and Significance of Cyclin E and p27kip1 in Gallbladder Carcinoma

Objective To evaluate the expression of cyclin E and p27 kip1 protein and their significance in gallbladder carcinoma. Methods SP immunohistochemistry was used to detect the expression of cyclin E and p27 kip1 protein in 41 cases gallbladder carcinomas,15 cases chronic cholecystitis tissues.Results The positive rate of cyclin E in gallbladder carcinoma was 61.0%(25/41),which was significantly higher than that in chronic cholecystitis (20.0%,3/15),P<0.05; The expression of cyclin E positively correlated with tumor TNM staging (r=0.314,P< 0.05). The positive rate of p27 kip1 in gallbladder carcinoma was 53.7%(22/41),which was lower than that in chronic cholecystitis (100%). The positive rate of p27 kip1 was decreased with the poor differentiation and progression of TNM staging. There was negative correlation between cyclin E and p27 kip1 expression (r=-0.342,P<0.05).Conclusion The high expression of cyclin E and the decreased expression of p27 kip1 result in abnomal regulation of cell cycle,which may be associated with gallbladder carcinogenesis and progression.