单克隆抗体和吡喹酮协同杀伤日本血吸虫的初步研究

昆明系小白鼠人工感染日本血吸虫尾蚴前２ｈ，４组口服低剂量吡喹酮（吡２０ｍｇ／ｋｇ或吡５０ｍｇ／ｋｇ），并被动免疫转移抗日本血吸虫单抗（ＭｃＡｂ１４或ＭｃＡｂ２４），其减虫率分别为４５．４０％（ＭｃＡｂ１４＋吡２０ｍｇ／ｋｇ）、４４．１５％（ＭｃＡｂ１４＋吡５０ｍｇ／ｋｇ）、４５．７４％（ＭｃＡｂ２４＋吡２０ｍｇ／ｋｇ）和６５．８７％（ＭｃＡｂ２４＋吡５０ｍｇ／ｋｇ），均高于２组被动免疫转移单抗组的减虫率［２９．８８％（ＭｃＡｂ１４）、４０．０１％（ＭｃＡｂ２４）］和２组单剂吡喹酮组的减虫率［１３．８５％（吡２０ｍｇ／ｋｇ）、３１．５０％（吡５０ｍｇ／ｋｇ）］。实验结果提示，单抗协同吡喹酮，可提高减虫率，对预防日本血吸虫病具有一定意义。

STUDIES ON MONOCLONAL ANTIBODIES AND PRAZIQUANTEL SYNERGICALLY KILLING SCHISTOSOMA JAPONICUM

Nine groups of Kunming mice were challenged with cercariae.Among them,groups 1 to 6 were fed with lower dosage of praziquantel 2h before challenged.At the same time,groups 1 to 4 and group 7 to 8 were immunized with 0.8ml McAb by peritoneal injection.Fourweeks after challenge,all groups of mice were killed and perfused. The perfusion results showedthat the worm reduction rates of groups 1 to 4 ranged from 44.15%to 65.87%,higher than thoseof single dose praziquantel groups,which ranged from 13.85%to 31.50%,also higher thanthose immunized with McAb groups,which ranged from 29.88%to 40.01%.The difterence ofworm reduction rates demonstrates that the synergy between praziquantel treatment and passivetransfered immunity with McAbs could enhance the killing rates against Schistosoma japonicum.