| 重组质粒pMG611中K99和F41抗原基因在E．coli中表达条件的研究 |
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| 引用本文： | 钟声 李丰生 等. 重组质粒pMG611中K99和F41抗原基因在E．coli中表达条件的研究[J]. 军事医学科学院院刊, 1991, 15(4): 282-284 |
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| 作者姓名： | 钟声 李丰生 等 |
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| 作者单位： | [1]佳木斯医学院生物教研室 [2]军事医学科学院生物工程研究 |
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| 摘 要： | 本文通过ELISA方法测定了重组质粒pMG611在不同宿主和培养条件下，K99和F41菌毛抗原的表达水平。研究结果表明重组质粒pMG611在HB101和RRI宿主菌中较在C600宿主菌中表达更多的K99和F41菌毛抗原。实验中还探讨了培养基、温度和丙氨酸对两种菌毛抗原表达的影响。
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| 关 键 词： | K99菌毛 F41菌毛 重组质粒 pMG611 K99 |
THE EXPRESSION CONDITION OF RECOMBINANT PLASMID pMG611 FOR K99 AND F41 FIMBRIAE ANTIGENS |
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| Abstract: | The expression levels of recombinant plasmid pMG611 for K99 and F41 fimbriae antigens in different hosts and under different incubation conditions were measured by ELISA.The results indicated that recombinant plasmid pMG611-harboring strains HB 101 and RRI produced more fimbriae antigens than that of C600 (pMG611).The effects of the media,temperatures and alanine to the expression of recombinant plasmid pMG611 for K99 and F41 fimbriae antigens were also studied. |
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| Keywords: | K99 antigen F41 antigen expression |
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