抗凝剂、标本保存温度和保存时间对流式T淋巴细胞亚群检测的影响

目的 调查抗凝剂、标本保存温度和保存时间对流式T淋巴细胞亚群检测结果的影响程度,为流式T淋巴细胞亚群检测标准化提供科学依据.方法 采用三色流式细胞术FSC/SSC设门策略分析健康体检人群外周血T淋巴细胞亚群,调查EDTA-K2和肝素锂两种抗凝剂以及标本保存温度与标本保存时间对检验结果的影响.结果 常温(18 ℃)保存的标本检测结果最为稳定,极端低温和极端高温都将引起检测结果的降低.EDTA-K2抗凝的标本18 ℃可至少保存3 d,肝素锂抗凝的标本18 ℃可保存2 d.EDTA-K2抗凝的标本检测结果较肝素锂抗凝的标本高.结论 流式T细胞亚群检测标本宜采用EDTA-K2抗凝,不能及时检测时宜18 ℃保存,冬季和夏季需要采取措施将标本的保存温度调节至常温为宜.

Empirical study of the influence of decoagulant,storage temperature and time on the detection of peripheral blood T-lymphocyte subgroups by flow cytometry

Objective To investigate the influences of decoagulant,storage temperature and time on the detection of peripheral blood T-lymphocyte subgroups by flow cytometry for providing scientific data to standardize the detecting procedure.Methods The contents of peripheral blood T-lymphocyte subgroups were detected by a 3-color flow cytometry with FSC/SSC gating for lymphocytes from health-determination persons.The blood samples,anti-coagulated by EDTA-K2 and heparin lithium respectively,were detected after being stored at different temperatures and different times.Results The results of samples,stored at common temperature (18 ℃) were more stable,and of samples,stored at too low or high temperature were decreased.The keeping time of samples,anti-coagulated by EDTA-K2 and stored at 18 ℃,was three days,but by heparin lithium was only two days.The results of samples,anti-coagulated by EDTA-K2,were higher than samples,anti-coagulated by heparin lithium.Conclusion It could be more favorable for the treatment of samples for T-lymphocyte detection by flow cytometry to be anti-coagulated by EDTA-K2 and stored at 18 ℃,if the detection could not be performed in time.During summer and winter,the storage temperature should be regulated to common temperature.