HPLC测定不同产地当归中阿魏酸的含量

目的：采用高效液相色谱法测定甘肃不同产地当归中阿魏酸含量,并对不同产地药材质量做客观比较。方法：用高效液相色谱法,使用ZORBAX Edipse XDB-C18柱（250 mm×4.6 mm,5μm）色谱柱;流动相：乙腈∶0.085%水溶液（17∶83）（V/V）;流速为1.0 mL/min;进样量为10μL;柱温为：35℃;检测波长为316 nm。结果：3个不同产地当归中阿魏酸含量有明显差异,岷县〉漳县〉哈达铺,其中岷县当归阿魏酸含量为0.1206%。3个不同产地当归阿魏酸含量均高于药典标准。结论：甘肃不同产地当归阿魏酸含量均高于药典标准,其中以道地产区岷县当归药材质量较好。

Determination of Fendic Acid Content in DangGui from Different Habitats by HPLC

Objective： To determine femlic acid content of DangGui from different habitats of Gansu by high performance liquid chromatography （HPLC） and to compare the quality of medicinal materials form different habitats objectively. Method： HPLC, the analytic column is ZORBAX Edipse XDB-C18 （250 min×4.6 mm, 5 μm）, mobile phase：acetonitrile：0.085% water（17：83）（V/V）; flow rate：l.0 mL/min; injection volume：10 μL; oven temperature：35℃; UV detection wavelength：316 nm. Result： Comparison of Ferulic acid contents of DangGui from three habitats showed significant difference, Minxian〉Zhangxian〉Hadapu, among them the highest ferulic acid content of DangGui from Minxian is 0.1206%. Ferulic acid contents of DangGui from three habitats are higher than the standard of Chinese traditional medicine Codex. Conclusion： Femlic acid contents of DangGui from three habitats are higher than the standard of Chinese traditional medicine Codex. Among them, DangGui of better quality is from Minxian.