HBV-M表达模式和HBV-DNA定量与原发性肝癌的关系研究

目的 探讨乙型肝炎病毒血清学标志物(HBV-M)表达模式、HBV-DNA复制水平与原发性肝癌(HCC)的关系.方法 216例原发性肝癌(HCC组),353例慢性乙型肝炎(CHB组),应用酶联免疫吸附试验和聚合酶链式反应方法分别检测两组血清HBV-M和HBV-DNA水平.结果 两组HBV-M表达模式构成差异有统计学意义(P＞0.05),HCC组血清学小三阳(HBsAg、HBeAb、HBcAb阳性)表达率(62.04%)显著高于大三阳(HBsAg、HBeAg、HBcAb阳性)表达率(18.98%);CHB组小三阳表达率为61.47%,大三阳为36.83%;CHB组大三阳表达率高于HCC组(P＜0.05).HBV-DNA阳性率HCC组为56.5%,CHB组为79.0%,差异无统计学意义(P＞0.05),但在HBV-DNA阳性患者中,HCC组HBV-DNA复制水平显著低于CHB组,差异有统计学意义(P＜0.05).结论 HCC患者HBV-M表达模式以小三阳为主,其HBV-DNA复制水平较CHB患者低.

An Investigation of the Relationship between the Development of Hepatocellular Carcinoma and the Expression Model of Hepatitis B Virus Serological Markers or the Level of HBV-DNA

Objective To explore the relationship between the development of hepatocellular carcinoma and the expression model of hepatitis B virus serological markers(HBV-M)or the replication level of hepatitis B virus DNA(HBV-DNA).Methods 216 cases of hepatocellular carcinoma were enrolled as case group(HCC group),and 353 patients with chronic HBV infection as the control group(CHB group),Enzyme-linked immunosorbent assay(ELISA)and polymerase chain reactiongroup(PCR)were used to detect the HBV-M and the replication level of HBV-DNA respectively.Results The rate of the model(HBsAg,HBeAb,HBcAb positive,STP)was 62.04% and 61.47% in the case group and the control group respectively(P>0.05),while the rate of the model(HBsAg,HBeAg,HBcAb positive,LTP)was 18.98% and 36.83% in the case group and the control group respectively(P>0.05).The rate of the model STP was significantly higher than the rate of the model LTP in the case group(P>0.05),but there was no significant difference in the control group.The rate of the model LTP in CHB group was also higher than that in HCC group(P<0.05).Positive rates of HBV-DNA in the patients with HCC and the patients with chronic HBV infection were 56.5% and 79% respectively.The replication level of HBV-DNA in HCC group(4.99±1.02)was significantly lower than that(5.80±1.43)of CHB group(P<0.05).Conclusion The expression model of HBsAg,HBeAb,HBcAb in patients with hepatocellular carcinoma is given priority to the patients with chronic HBV-infection,and the replication level of HBV-DNA of hepatocellular carcinoma is significantly lower than that of chronic HBV infection.