儿童传染性单核细胞增多症T细胞亚群的动态分析

目的 检测EB病毒感染引起传染性单核细胞增多症(IM)患儿T细胞亚群的细胞表达情况,探讨IM患儿的细胞免疫功能状况.方法 应用流式细胞术(FCM)检测30例IM患儿组急性期和恢复期外周血T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+的细胞表达率,并与随机抽取的24例同龄健康对照组儿童进行比较.结果 IM患儿组急性期CD3+、CD8+细胞表达率较对照组明显增高(P<0.05);而CD4+、CD4+/CD8+较对照组明显降低(P<0.05).IM患儿组恢复期CD3+、CD8+细胞表达率较急性期有所降低,但仍高于健康对照组(P<0.05);恢复期CD4+、CD4+/CD8+较急性期有所增加,但仍低于对照组(P<0.05).结论 IM患儿急性期外周血T淋巴细胞亚群明显异常,导致机体免疫功能改变是IM发病的主要原因之一,监测IM患儿的细胞免疫功能状况对疾病的预后有重要的临床意义.

Dynamic analysis of T lymphocyte subsets in children with infectious mononucleosis

Objective To investigate the immunity function in children with infectious mononucleosis(IM),caused by epstein-barr virus(EBV) infection,by measuring T lymphocyte subsets in peripheral blood .Methods Flow cytometry (FCM) technique was applied to quantitatively analyze CD3+,CD4+,CD8+ and CD4+/CD8+ T lymphocyte subsets in peripheral blood of 30 case of child patients with IM at acute or convalescent stage and 24 cases of healthy controls.Results Compared with control group,positive rates of CD3+ and CD8+ T lymphocyte subsets in child patients at acute phase were obviously increased(P<0.05),while CD4+ and CD4+/CD8+ were significantly decreased(P<0.05).Compared with child patients at acute stage,positive rates of CD3+ and CD8+ in child patients at convalescent stage were obviously decreased(P<0.05),which were still higher than control group(P<0.05),but CD4+ and CD4+/CD8+ were significantly increased(P<0.05),which were still lower than control group(P<0.05).Conclusion The variation of immune function,characterized by obvious abnormality of T lymphocyte subsets in peripheral blood at acute stage,could be one of the main causes of IM in child patients.There is vital clinical significance to monitor the immunity function in for the prognosis of IM in child patients,