强直性脊柱炎患者外周血淋巴细胞亚群分析

目的 了解强直性脊柱炎患者外周血淋巴细胞亚群变化规律,探讨患者体内的免疫功能状态.方法 采用三色流式细胞学检测技术分析75例患者及50例健康对照的外周血T细胞亚群、B细胞与NK细胞水平,并对其结果进行比较分析.结果 强直性脊柱炎组总T细胞、T4细胞、T8细胞的百分含量依次为(65.74±9.99)%、(35.70±6.92)%和(23.02±5.35)%;T4/T8比值1.70±0.51,总T细胞、T4细胞、T8细胞的绝对含量依次为(1.25±0.63)、(0.47±0.27)和(0.31±0.19)×109/L.B细胞和NK细胞的百分含量分别为(11.84±4.50)%和(15.43±6.28)%;B细胞和NK细胞的绝对含量分别为(0.22±0.15)和(0.27±0.15)×109/L.与健康对照组比较,患者总T细胞、B细胞和NK细胞绝对含量增加(P＜0.01或P＜0.05),但各淋巴细胞亚群的百分含量无明显改变(P>0.05).与腰背痛组、B27+无临床症状组、B27+非强直组比较,各淋巴细胞亚群的百分含量和绝对含量均略有增高,但差异无统计学意义(P>0.05).结论 强直性脊柱炎患者机体免疫功能存在一定程度的紊乱,且以总T细胞、B细胞、NK细胞功能亢进为特点.临床腰背痛患者、B27+无症状者、B27+非强直患者均表现出与临床强直患者相似的免疫功能紊乱.

Analysis of lymphocyte subsets in peripheral blood in patients with ankylosing spondylitis

Objective To study the alteration regularity of peripheral blood lymphocyte subsets in patients with ankylosing spondylitis(AS),and investigate their immune functional status.Methods Peripheral blood lymphocyte subsets were detected by 3-color flow cytometry in 75 patients (AS group) and 50 healthy subjects (control group).The results were analyzed statistically.Results In AS group,the percentages of T lymphocyte,CD4+T lymphocyte and CD8+ lymphocyte as well as the ratio of CD4+/CD8+ were (65.74±9.99)%,(35.70±6.92)%,(23.02±5.35)% and 1.70±0.51,and the absolute quantities of T lymphocyte,CD4+T lymphocyte and CD8+T lymphocyte were (1.25±0.63),(0.47±0.27) and (0.31±0.19)×109/L.The percentages of B lymphocyte and NK lymphocyte were (11.84±4.50)% and (15.43±6.28)%,and the absolute quantities of each of them were(0.22±0.15) and (0.27±0.15)×109/L.Compared with the control group,the absolute quantities of T lymphocyte,B lymphocyte and NK lymphocyte in AS group were significantly higher(P<0.01 or P<0.05),but according to percentage of different cells,there was no statistical difference(P>0.05).Compared with lumbodorsal pain group,group positive with B27 but negative with clinical symptom and non-AS group positive with B27,the percentages and absolute quantities of all lymphocyte subsets in AS group were clearly higher,but with no significant difference(P>0.05).Conclusion There might be immune function disorder in patients with AS.In lumbodorsal pain patients,patients positive with B27 but negative with clinical symptom and non-AS patients positive with B27,there could be similar immune function disorder.