海拔4500m地区藏、汉人群外周血淋巴细胞亚群检测

目的探讨高原缺氧对机体免疫功能及其免疫调节功能的影响。方法采用流式细胞术对高海拔地区(4 500 m)52名健康汉族人群和35名健康藏族人群进行了淋巴细胞亚群CD4+、CD8+、CD3-CD19+(B细胞)、CD3-CD56+(NK细胞)测定,并与中度海拔(西宁)地区45名健康汉族人群进行了比较。结果 2个海拔的CD4+、CD8+、CD3-CD19+、CD3-CD56+各项中,4 500 m人群CD8+高于西宁组,CD4+明显低于西宁组,P<0.05;CD19+(B细胞)西宁组高于高海拔组,P<0.05;海拔4 500 m汉族、世居藏族人群的CD4+、CD8+无统计学意义比较,P>0.05;CD3-CD56+(NK)藏族低于汉族,P<0.05;CD3-CD19+(B细胞)藏族高于汉族,P<0.05。结论高原低氧环境影响机体的免疫调节功能。

Level of peripheral blood lymphocyte subsets of Tibetan and Han populations in an altitude of 4 500 meters

To investigate the impact of high altitude hypoxia on immune function,the lymphocyte subsets of CD4+,CD8+,CD3-CD19+(B cells) and CD3-CD56+(NK cells) of 52 healthy Han Chinese and 35 healthy Tibetan people in high-altitude areas(4 500 m) were detected using the flow cytometry,and compared them with 45 healthy Han Chinese in middle-altitude areas(Xining area).We found that the level of CD8+ in 4 500 m area groups was higher than that of Xining group,but CD4+ is obviously lower than that of the Xining group(both P < 0.05);the level of CD19+(B cells) in Xining group is higher than that of the other group in high-altitude areas(P < 0.05.There is no statistical significance of CD8+ and CD4+ levles in Tibetan group and Han group of high altitude area(P < 0.05),while the level of CD3-CD19+(B cells) in Tibetan group was higher than that in Han group of high altitude area(P < 0.05).We get the conclusion that lymphocyte subsets of the indicators are part of change in the environment of high altitude hypoxia.