| 反义p21WAF1/CIP1基因转染对肾癌细胞凋亡抑制作用的影响 |
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| 引用本文： | 刘贺亮,崔大祥,王禾,陈宝琦,邵国兴,严泉剑. 反义p21WAF1/CIP1基因转染对肾癌细胞凋亡抑制作用的影响[J]. 医学争鸣, 2002, 23(6): 527-529 |
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| 作者姓名： | 刘贺亮 崔大祥 王禾 陈宝琦 邵国兴 严泉剑 |
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| 作者单位： | 1. 第四军医大学西京医院泌尿外科,陕西,西安,710033
2. 第四军医大学全军基因诊断技术研究所,陕西,西安,710033 |
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| 摘 要： | 目的 探讨p21^WAF1/CIP1基因在顺铂诱导的GRC-1肾癌细胞凋亡过程中的作用。方法 使用脂质体转染方法将正义p21^WAF1/CIP1基因及反义p21^WAF1/CIP1基因基因分别导入GRC-1肾癌细胞系中，对所获转染细胞进行形态学观察和流式细胞仪检测。结果 在正义p21^WAF1/CIP1基因基因转染后GRC-1肾癌细胞中，顺铂诱导细胞凋亡的作用增强；在反义p21^WAF1/CIP1基因基因转染后GRC-1肾癌细胞中，顺铂诱导细胞凋亡的作用受到抑制。结论 在GRC-1肾癌细胞中，顺铂诱导的细胞凋亡至少部分是通过p21^WAF1/CIP1基因基因发挥作用。p21^WAF1/CIP1基因基因作为一个与细胞凋亡的相关基因可能参与肾癌细胞凋亡的调控。
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| 关 键 词： | 原癌基因蛋白质p21 转染 脱噬作用 细胞凋亡 肾肿瘤 肿瘤细胞 |
| 文章编号： | 1000-2790(2002)06-0527-03 |
| 修稿时间： | 2001-10-30 |
Effect antisense p21WAF1/CIP1 gene on renal carcinoma cell apoptosis |
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| LIU He-Liang ,CUI Da-Xiang ,WANG He ,CHEN Bao-Qi ,SHAO Guo-Xing ,YAN Quan-Jian. Effect antisense p21WAF1/CIP1 gene on renal carcinoma cell apoptosis[J]. Negative, 2002, 23(6): 527-529 |
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| Authors: | LIU He-Liang CUI Da-Xiang WANG He CHEN Bao-Qi SHAO Guo-Xing YAN Quan-Jian |
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| Affiliation: | LIU He-Liang 1,CUI Da-Xiang 2,WANG He 1,CHEN Bao-Qi 1,SHAO Guo-Xing 1,YAN Quan-Jian 1 1Department of Urolog Surgery,Xijing Hospital,2Institute of Gene Diagnosis of Chinese PLA,Fourth Military Medical University,Xi'an 710033 |
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| Abstract: | AIM To explore effect of p21 WAF1/CIP1 gene on GRC-1 renal carcinoma cell apoptosis mediated by Cisplatin. METHODS p21 WAF1/CIP1 and antisense p21 WAF1/CIP1 were respectively transfected into GRC-1 renal carcinoma cell line byusing lipofectin method. The observation of morphology and the detection of flow cytometer were made on transfected GRC-1 cells. RESULTS Apoptosis mediated by Cisplatin were reinforced on p21 WAF1/CIP1 gene transfected GRC-1 cells,but were inhibited on antisense p21 WAF1/CIP1 gene transfected GRC-1 cells. CONCLUSION p21 WAF1/CIP1 gene at least partly produces a marked effect on apoptosis mediated by Cisplatin. p21 WAF1/CIP1, as a gene closely related to apoptosis, might modulate the death or survivorship of renal carcinoma cell. |
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| Keywords: | proto-oncogene protein p21(ras) transfection apoptosis kidney neoplasms tumor cells cultured |
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