阿霉素诱导慢性充血性心力衰竭模型制作的改良方案

目的建立阿霉素心肌病（Adr-DCM）动物模型，并对其进行评价。方法Adr-DCM模型建立方法：阿霉素2mg／kg，腹腔注射，前5次为每3天注射，后5次每7天注射，累计用量为20mg／kg。应用超声心动图检测心功能指标，电子显微镜评价心肌超微结构。结果阿霉素应用4周后即存在心肌超微结构改变，10周后大鼠心力衰竭诱导成功率100％，死亡率25％。结论阿霉素多次腹腔注射可以导致大鼠心肌重构和心功能异常，通过改良给药方法，动物死亡率明显降低，可以作为心肌病模型制作的新方法推广。

A Modified Rat Model of Dilated Cardiomyopathy Induced by Adriamycin

Objective To establish a more effective rat model of dilated cardiomyopathy induced by adrimycin.Methods Fourty-two adult male Wistar rats were randomly divided into 2 groups.Adrimycin was injected into the rats of the Adr-DCM group by intraperitoneal at the dose of 2 mg/kg per 3 days for 5 times;then at a dose of 2 mg/kg per 7 days for 5 times.while rats of the NC group were injected with 0.9% saline using the same methods.Transthoracic echocardiography was applied to evaluate rats heart function and electronmicroscope was applied to evaluate rats morphology of myocardium.Results Compared to the control group,LV end diastolic diameter(LVEDD) and LV end systolic diameter(LVESD) of rats in Adr-DCM group were both significantly increased(P<0.05),while the LV ejection fraction(EF) and LV fraction shortening(FS) were significantly decreased(P<0.05) in Adr-DCM group 10 weeks after administration of adriamycin.The cumulative mortality in Adr-DCM group was 25%.Conclusions Chronic administration of intraperitoneal injection adriamycin to rat results in a dialted cardiomyopathy;a more effective rat model of a dilated cardiomyopathy induced by drimycin is established.