Effect of carcinogenic polycyclic aromatic hydrocarbons on mouse embryonic cells in culture: Induction of spindle-shaped cells |
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Authors: | Hiroya Kamei |
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Institution: | Central Research Institute, The Japan Tobacco and Salt Public Corporation, 6-2 Umegaoka, Midori-ku, Yokohama, Kanagawa, 227 Japan |
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Abstract: | In cultured mouse embroyonic cells (MECs) treated with benzoa]pyrene(Ba]P), there appeared unusual type of fibroblasts, spindle-shaped cells (SP cells), which were characterized by their narrow bipolar shape, long cellular processes and optically distinct cell borders. Appearance of SP cells was massive and irreversible. The amount of SP cells increased with increased with increasing concentrations of Ba]P, while early cytotoxicity did not. In various polycyclic aromatic hydrocarbons (PAHs) tested, only potent carcinogens {7,12-dimethylabenza]anthracene (DMBA), 3-methylcholanthrene (MCA), Ba]P and dibenza, e]pyrene (DBa, e]P)} induced SP cells. Among them, PAH having higher Iball's index induced SP cells at lower concentration and at an earlier time. Weak or non-carcinogenic PAHs including 3-hydroxybenzo-a]-pyrene(3-H-Ba]P) did not induce SP cells. α-Napthoflavon (αNF) suppressed the induction of SP cell by carcinogenic PAH. SP cells did not appear spontaneously under various abnormal culture conditions. These results indicate that carcinogenic PAHs induce the appearance of a specific type of fibroblast, SP cells in MEC cultures in accordance with their carcinogenicity. |
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Keywords: | MEC mouse embryonic cell SP cell spindle-shaped cell PAH polycyclic aromatic hydrocarbon B[a]P benzo[a]pyrene MCA 3-methylcholanthrene DMBA 7 12-dimethylbenz[a]anthracene DB[a e]P dibenz[a e]pyrene DB[a h]A dibenz[a h]-anthracene CR Chrysene DB[de kl]A dibenz[de kl]anthracene FL fluoranthene PY pyrene 3-OH-B[a]P 3-hydroxybenzo[a]pyrene αNF α-naphthoflavon DMSO dimethylsulphoxide AHH arylhydrocarbon hydroxylase cAMP adenosine 3′ 5′-cyclic monophosphate HEPES 4- or 6-NQO 4- or 6-nitroquinoline-1-oxide MNNG |
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