Characterization of three different human T cell membrane antigens,two being present on T lymphocyte subpopulations |
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Authors: | RJ Billing B Clark PI Terasaki |
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Institution: | From the Department of Surgery, UCLA School of Medicine, University of California, Los Angeles, California, USA |
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Abstract: | Heteroantisera were raised in rabbits to thymocytes, HSB2 cells, and Sezary cells. Following absorption with Ia-positive leukemia cells, these sera appeared to be specific for different T cell antigens. Both the anti-HSB2 and the anti-Sezary sera reacted with approximately 50% and the antihymocyte serum with 100% of normal peripheral blood T lymphocytes. None of the sera reacted with B cells. The apparent molecular weights of the antigens being derected were determined by immunoprecipitation followed by SDS polyacrylamide gel eletrophoresis. A dimer of 170,000 daltons consisting of two similar 85,000-dalton polypeptide chains was immunoprecipitated by the anti-HSB2 serum whereas single polypeptides of 53,000 and 64,000 daltons were immunoprecipitated by the anti-Sezary and antithymocyte sera, respectively. |
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Keywords: | SRBC sheep red blood cells ALS antilymphocyte serum AML acute myelocytic leukemia DTT dithiothreitol ALL acute lymphocytic leukemia |
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