局部应用bFGF对大鼠带真皮下血管网皮片的影响

目的:探讨局部应用bFGF浸泡移植大鼠带真皮下血管网皮片,对皮片成活率的影响,为临床提高全厚皮片移植的成功率提供实验依据和临床依据。方法:SD大鼠1 0只,随机分为2组,每组5只。于每只大白鼠背部前后相对固定的位置剪取2片直径约3cm带真皮下血管网的皮片(包括背部肉膜层,在背部肌膜的浅面进行) ,第1组前侧皮片即为实验组,将所取皮片应用贝复济( 36 0 0 0AU/1 5mLbFGF)浸泡1 0min后,回植原位,周边间断缝合,并打包固定。第1组后侧所取皮片为对照组,所取皮片应用生理盐水浸泡1 0min后,回植原位,周边间断缝合,并打包固定。第2组大鼠以后面所取皮片为实验组,而以前面所取皮片为对照组,其余处理同前。术后5d ,将大白鼠处死,肉眼观察皮片成活情况,取材组织学检查毛细血管数目及肉芽组织生长情况。结果:实验组与对照组皮片成活率依次为95%和72 %。经配对t检验统计学处理有显著性差异(t =3 .4 53,P <0 .0 1 )。镜下毛细血管数量有明显差异(t =2 . 51 ,P <0 . 0 5)。肉芽组织生长厚度有显著差异(t=3 .30 7,P <0 . 0 1 )。结论:局部应用bFGF可促进新生血管形成对提高皮肤移植的成活率有重要的临床意义。

Influence of Basic Fibroblast Growth Factor by Local Application Method (Soak) on the Healing of Subcutaneous Vascular Net Free Skin Graft in Rats

Objective: To study the influence of basic fibroblast growth factor (bFGF) by local application methed (soak) on the healing of remove subcutaneous vascular net free skin graft in rats .Metheds: 10 SD rats,(ordinary experiment animals ,female and male ,body weight 275g～285g) randomly divided into two bundles. Two skin grafts of subcutaneous vascular net were cut off in the back respectively and then transplanted in situs.The upside skin grafts were used were as test group,free skin graft soak 10 minuts in bFGF, and downside skin grafts were used as control group, free skin graft soak 10 minuts in NaCl . After operation, the survival of skin grafts was generally observed by naked eye. the skin grafts were sampled to observe numbers of blood capillary and growth of granulation tissues with microscope.Results: The survival of skin grafts in the test and control groups were 95% and 72% respectively ( t =1.753, P <0.01) .Under the microscope, the surviving area of experiment bundle had a great deal granulation tissues, a large number newly born blood vessels, distinctly below muscular layer. There were significant differences in the numbers of blood capillary and growth of granulation tissues between the two groups ( P <0.05) . There were significant differences in growth of granulation tissues ( P <0.01).Conclusion: bFGF,as an important vascularization factor , has a distinc auxo-action to the surviving of removed free skin graft.but also the comprehensive effect on cell proliferation . Local application may accelerate new vascularization.There was an important clinical significance in rising rate of tissue graft.