| 高效液相色谱法测定中药制剂中齐墩果酸的含量 |
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| 引用本文： | 游勇基,张桂凤.高效液相色谱法测定中药制剂中齐墩果酸的含量[J].海峡药学,2009,21(7):67-69. |
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| 作者姓名： | 游勇基 张桂凤 |
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| 作者单位： | 1. 福建省药品检验所,福州,350001
2. 福建医科大学,福州,350001 |
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| 摘 要： | 目的 建立测定中药制剂中有效成分齐墩果酸含量的方法。方法 采用高效液相色谱法测定。以YMC C18柱（150mm×4．6mm，5μm）为色谱柱。乙腈-甲醇-0．5％醋酸铵溶液（67：12：21）为流动相,检测波长为215nm，柱温为室温,用外标峰面积法计算。结果 齐墩果酸在0．8832-8．832μg范围内呈良好线性关系，回归方程为Y=207．65X＋8．0064（r=1．0000）．齐墩果酸的平均回收率为96．46％（RSD=1.13％，n=6）。结论 方法简便，分离效果好，结果准确可靠，可以用于中药制剂的质量控制。
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| 关 键 词： | 齐墩果酸 高效液相色谱法 |
Determination of oleanolic acid in traditional chinese medicine by HPLC |
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| YOU Yong-ji,ZHANG Gui-feng.Determination of oleanolic acid in traditional chinese medicine by HPLC[J].Strait Pharmaceutical Journal,2009,21(7):67-69. |
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| Authors: | YOU Yong-ji ZHANG Gui-feng |
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| Institution: | YOU Yong-ji, ZHANG Gui-feng(Fujian Institute for Drug Control, Fuzhou 350001 China) |
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| Abstract: | OBJECTIVE To establish a method for determination of oleanolic acid in traditional chinese medicine. METHODS The analysis was carried on a column of YMC C18 （150 × 4.6mm, 5μm）with a mobile phase of acetonitrile-methanol-0.5 % ammonium acetate solution（67： 12：21）. The detection wavelength was at 315nm. RESULTS The linear regression equation was Y = 207.65X ＋ 8. 0064（r = 1. 0000） for oleanolic acid and the linear range was 0. 8832-8. 832μg. The average recoveries was 96.46 % （RSD = 1.13 %, n = 6）for oleanolic acid. CONCLUSION This is a rapid , simple and accurate method which can be used to control the qulity of traditional chinese Medicine. |
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| Keywords: | Oleanolic acid HPLC |
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