| 贵阳地区汉族D12S375位点基因频率调查 |
| |
| 引用本文： | 张红玲 单可人. 贵阳地区汉族D12S375位点基因频率调查[J]. 贵阳医学院学报, 1999, 24(4): 334-336 |
| |
| 作者姓名： | 张红玲 单可人 |
| |
| 作者单位： | [1]贵阳医学院法医学教研室 [2]贵阳医学院分子生物学研究室 |
| |
| 基金项目： | 贵阳医学院青年教师基金 |
| |
| 摘 要： | 应用ＰＣＲ技术和聚丙烯酰胺凝胶垂直电泳技术对Ｄ１２Ｓ３７５位点分型，在贵阳地区１０６位汉族个体中共检出６个等位基因，１８种基因型，基因型频率分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡（Ｐ〉０．０５），１３频率最高，为０．４１５１；１６频率最低，为０．０１８８。观察杂合度０．７０，个人识别机率０．８９，非父排除率０．４９３。表明该位点是一个较好的ＤＮＡ遗传标记系统，在法医学亲子鉴定及个人识别中具有较高
|
| 关 键 词： | D12S375 等位基因 基因频率 法医学 DNA |
A Study Of Han Population Allele Frequency of D12S375 Locus in Guiyang Area |
| |
| Zhang Hongling,et al. A Study Of Han Population Allele Frequency of D12S375 Locus in Guiyang Area[J]. Journal of Guiyang Medical College, 1999, 24(4): 334-336 |
| |
| Authors: | Zhang Hongling et al |
| |
| Affiliation: | Zhang Hongling,et al Department of Forensic Medicine,Guiyang Medical College |
| |
| Abstract: | Using the polymerase chain reaction (PCR), D12S375 was amplified on DNA samples extracted from EDTA-blood of 106 unrelated individuals Han nationality in Guiyang. The PCR products were analyzed by polyacrylamide gel (PAG) vertical electrophoresis. 6 alleles and 18 genotypes were found at D12S375 locus, the distribution of genotypes revealed no deviation from the Hardy-weinberg equilibrium(P> 0.05). The frequency of allele 13 is the highest, which is 0.415 1,and that of allele 16 is the lowest, which is 0.018 8. The observed heterozygosity was 0 70, the power of discrimination was 0.89, and the chance of paternity exclusion was 0.493. The results of this study show that D12S375 locus is a useful DNA genetic marker system for personal indentification and paternity test in the fields of forensic science. |
| |
| Keywords: | D12S375 alleles Gene frequency |
| 本文献已被 CNKI 维普 等数据库收录！ |
