红景天与熟地配伍对骨髓抑制小鼠骨髓功能的影响

目的 探讨红景天与熟地配伍对环磷酰胺所致小鼠外周血白细胞下降及骨髓造血功能抑制的

Effect of Combined Use of Rehmannia and Rhodiola on Hemopoietic Function in Mice with Bone Marrow Depression

Objective To explore the effect of combined use of rehmannia（RM） and rhodiola（RD） on peripheral leukopenia and bone marrow hematopoietic function suppression induced by cyclophosphamide（CTX） in mice.Methods ICR mice were established into bone marrow inhibition models by intraperitoneal injection of CTX,and were administered with RM,RD or its extract（RDE）,singly or in mixture,via gastrogavage for 10 days.The changes of peripheral hemogram,bone marrow nucleated cell proliferation,CFU-GM colony formation,GM-CSF and erythropoietin（EPO） secretion were observed.Results Compared with the un-treated model mice,the peripheral white blood cell count was significantly higher in model mice treated with RDE and RM mixture;the bone marrow nucleated cells count,CFU-GM formation,and GM-CSF production were significant higher in model mice treated with RD and RM mixture,showing statistical significance（P0.01）;while EPO production in the RD and RM mixture treated group was slightly elevated,but the difference showed no statistical significance.Conclusion RD and RM mixture could regulate hematopoietic system by promoting the production of bone marrow cells and colonies,as well as enhancing the synthesis of related cytokines,such as GM-CSF,so as to increase the amount of peripheral white blood cells and restore the hematopoietic function of organism.