IL-13在盆腔子宫内膜异位症患者血清和腹腔液中的表达及意义

目的 通过检测及探讨子宫内膜异位症（EMs）患者血清及腹腔液中IL-13水平的变化,初步推测其在EMS发病中的作用.方法 采用酶联免疫吸附试验（ELISA）,检测EMs组32例患者和对照组20例非EMs患者腹腔液及血清中IL-13的水平,比较两组之间的差异;并将EMs组血清及腹水IL-13水平分别与EMs R-AFS评分进行相关性分析.结果 EMs组血清IL-13水平（6.95±2.74）pg/ml]明显低于对照组（12.87±9.70） pg/ml]（P＜0.01）;EMs组腹腔液IL-13水平（3.26±1.81）pg/ml]明显低于对照组（8.49±6.65）pg/ml]（P＜0.01）.根据期别将EMs组分为轻度亚组（Ⅰ、Ⅱ期）和重度亚组（Ⅲ、Ⅳ期）,血清IL-13水平轻度亚组（7.85±2.09）pg/ml]与对照组（12.87±9.70） pg/ml]及轻度亚组（7.85±2.09 ）pg/ml]与重度亚组（6.34±3.00） pg/ml]均无统计学差异,重度亚组（6.34±3.00）pg/ml]明显低于对照组（12.87±9.70）pg/ml]（P＜0.05）;腹腔液IL-13水平重度亚组（2.43±1.43）pg/ml]明显低于轻度组（4.48±1.63）pg/ml]（P＜0.01）,后者又显著低于对照组（8.49±6.65）pg/ml]（P＜0.05）.EMs组腹腔液IL-13表达水平与其R-AFS评分呈负相关（r=-0.52,P＜0.05）.结论 EMs患者腹腔液中IL-13的水平显著降低,且与疾病的严重程度成负相关,推测IL-13在EMs的发病中起重要的作用.

IL-13 levels in serum and peritoneal fluid of endometriosis

Objective To determine the IL-13 levels in serum and peritoneal fluid of endometriosis. Methods IL-13 levels in peritoneal fluid and serum were detected in 32 patients with endometriosis（ EM ） and 20 controls by enzyme-linked immunosor- bent assay（ELISA）, the correlation of IL-13 level with RevisaI-American Fertility Score（R-AFS） and the correlation between serum and peritoneal fluid IL-13 levels were analyzed. Results IL-13 levels in serum and peritoneal fluid were significantly lower than that in control group （ 6.95 ± 2.74 ）vs（ 12.87 ± 9.70 ）pg/ml, P〈0.01; （ 3.26 ± 1.81 ）vs （8.49 ± 6.65） pg/ml, P〈0.01, respectively]. EMs group has been divided into mild（stage I and II ） and severe group（stage III and IV ） according to the stage of EMs. There were no differences in serum IL-13 levels between control group and mild EM group （ 12.87 ± 9.70） vs （7.85 ± 2.09 ）pg/ml, P 〉 0.05] and between mild EM group and severe EM group（ 7.85 ± 2.09） vs（6.34 ± 3.00）pg/ml, P 〉0.05]; however, IL-13 levels in severe EM group was significant lower than that in control group （ 6.34± 3.00 ） vs （ 12.87 ± 9.70 ）pg/ml, P〈0.05]. IL- 13 level in peritoneal fluid of severe EM group was significant lower than that in mild group（ 2.43 ± 1.43 ） vs （ 4.48 ± 1.63 ）pg/ml, P〈0.01], and IL-13 level in mild group was significant lower than that in control group （ 4.48 ± 1.63） vs （8.49 ± 6.65 ）pg/ml, P〈0.05]. IL-13 level in peritoneal fluid was negatively correlated with R-AFS （r=-0.52,P〈0.05）. Conclusion IL-13 level in peritoneal fluid of EM pa- tients is lower than that of controls and negatively correlated with the severity of EMS.