| 斑蝥天灸对环磷酰胺小鼠造血功能的影响及其机理探讨 |
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| 引用本文： | ?????,?o??,?????,????,??к?,?′??,??.斑蝥天灸对环磷酰胺小鼠造血功能的影响及其机理探讨[J].针灸推拿医学(英文版),2011,9(6):340-345. |
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| 作者姓名： | ????? ?º?? ????? ???? ??к? ?´?¡ ?? |
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| 作者单位： | [1]Acupuncture Department, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P. R. China [2]Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, E R. China |
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| 基金项目： | Shanghai Leading Academic Discipline Project （S30304） |
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| 摘 要： | 目的:观察斑蝥天灸对化疗小鼠造血功能和腹腔巨噬细胞分泌粒细胞-巨噬细胞集落刺激因子(Granulocyte-Macrophage Colony-Stimulating Factor,GM-CSF)能力的影响.方法:将环磷酰胺(Cyclophosphamide,CTX)小鼠模型随机分为6组,对照组10只不予任何治疗；天灸I-Ⅳ组分别采用不同浓度的斑蝥酊灸大椎、肾俞、足三里；麦粒灸组10只用精制艾绒制备的小艾炷灸上述穴位；另设正常组10只作对照.检测不同时期小鼠外周血白细胞、骨髓有核细胞数、脾指数及腹腔巨噬细胞诱生GM-CSF的能力.结果:CTX化疗可致小鼠出现明显的骨髓抑制,骨髓有核细胞计数和外周血白细胞(White BloodCell,WBC)数显著减少,脾指数减少,且其腹腔巨噬细胞诱生GM-CSF能力降低.天灸大椎、肾俞、足三里穴,不但可以不同程度地减轻骨髓抑制,促进骨髓增生活跃,提高骨髓有核细胞计数,提前回升外周血-WBC数,缩短化疗所致WBC的持续低值期,还可增强化疗小鼠巨噬细胞诱生GM-CSF的能力.结论:斑蝥天灸对化疗小鼠具有抗化疗骨髓抑制作用,能够增强化疗小鼠腹腔巨噬细胞诱生GM-CSF的能力.
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| 关 键 词： | 灸法 发泡疗法 环磷酰胺 药物疗法 粒细胞-巨噬细胞集落刺激因子 肿瘤 小鼠 |
Effect and mechanism of Cantharides vesiculation moxibustion on hematopoietic function in mice treated with cyclophosphamide |
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| Deli Sun,Hanping Chen,Huangan Wu,Yan Zhang,Chenghao Ni,Dalong Chen,Jian Pei.Effect and mechanism of Cantharides vesiculation moxibustion on hematopoietic function in mice treated with cyclophosphamide[J].Journal of Acupuncture and Tuina Science,2011,9(6):340-345. |
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| Authors: | Deli Sun Hanping Chen Huangan Wu Yan Zhang Chenghao Ni Dalong Chen Jian Pei |
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| Institution: | 1. Acupuncture Department, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P R. China
2. Shanghai Research Institute of Acupumcture and Meridian, Shanghai 200030, P. R. China |
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| Abstract: | Objective: To observe the effects of Cantharides vesiculation moxibustion on hematopoietic function and granuloeyte-macrophage colony-stimulating factor (GM-CSF) secreted by peritoneal macrophages in mice treated with chemotherapy.Methods: The mice treated with Cyclophosphamide (CTX) were randomly divided into 6 groups.Ten mice in the control group were not treated.The mice in the vesiculation Ⅰ-Ⅳ groups received moxibustion at Dazhui (GV 14),Shenshu (BL 23) and Zusanli (ST 36) with different concentrations of Cantharides tincture.The mice in the grain moxibustion group were treated with moxa cone of wheat-grain size at these points.The other 10 normal mice were adopted as control.The peripheral blood leukocytes count,bone marrow nucleated cells count,spleen index and the ability of peritoneal-macrophages-induced GM-CSF were detected at different stages.Results: CTX could cause significant bone marrow suppression,significant decrease of bone marrow nucleated cell counts,peripheral blood leukocytes and spleen index,and reduction of peritoneal macrophages induced GM-CSF in the mice.Vesiculation moxibustion at Dazhui (GV 14),Shenshu (BL 23) and Zusanli (ST 36),not only reduced the bone marrow suppression,promoted bone marrow hyperplasia,increased bone marrow nucleated cell count,enhanced the number of the peripheral blood white blood cell (WBC) and shorted low sustained period of WBC chemotherapy-induced,but also enhanced the ability of macrophage induced GM-CSF Conclusion: Cantharides vesiculation moxibustion has good resistance to the bone marrow suppression in mice treated with chemotherapy,and enhance the ability of the mouse peritoneal rnaerophages induced GM-CSF. |
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| Keywords: | Moxibustion Therapy Blistering Therapy Cyclophosphamide Drug Therapy GranulocyteMacrophage Colony-Stimulating Factor Neoplasms Mice |
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