小鼠触须毛囊体外培养的研究

目的 为了建立较理想的小鼠触须毛囊体外培养模型，对不鼠触须毛囊体外培养方法进行了研究。方法 通过小鼠触须毛囊体外无血清培养基的方法，对不同鼠龄及不同生长周期的毛囊进行了研究。结果 ①毛囊在Ｗｉｌｌｉａｍｓ－Ｅ加Ｌ－谷酰胺培养基上生长平均达８天；②通过对比不同阶段的生长期毛囊发现生长期－Ⅱ毛囊生长力明显优于其它各期毛囊；③对不同鼠龄毛囊体外增培养生长状况的研究发现出生３５天和６５天鼠优于出生７天乳鼠

Study on Organ Culture of Mouse Vibrissa Follicle in Vitro

Objective To study and establish a culture model of mouse vibrissa follicle in vitro Methods Mouse vibrissa follicles cultured in serum free medium were used to study the follicles in different ages of mice and in different growing stages Results 1 With Williams medium E and L glutamine, the follicles kept growing for more than 8 days 2 Follicles in the early phase of anagen (anagen II)grew significantly faster than the follicles in any other phases , 3 The growth of hair fibers was better in adult mice than that in 7 day old mice 4 The follicles after excising the upper one third grew faster than a mixture of two parts of cut follicles and the intact follicles Conclusion 1 Mouse vibrissa follicles in early anagen grew better than that in any other phases of the hair cycle 2 With Williams medium E plus L glutamine, the mouse follicles kept growing over 8 days 3 Cutting off the upper one third of the follicle during the culture promotes the growth of the follicle