皮肤源性前体细胞的分离及其向神经系统细胞的诱导分化研究

目的:观察可否从截瘫病人皮肤中分离到皮肤源性前体细胞(skin-derived precursors,SKPs)并诱导成神经系统细胞.方法:手术时从胸椎骨折伴或不伴截瘫病人切口边缘取2 cm2皮肤,剪成1～2 mm3碎片胰酶消化4 h.去除表皮层,吹打后过滤离心收集细胞进行培养.细胞荧光组织化学检测其细胞表型,并进行向...

Isolation and induction of differentiation into nervous system cells of the skin-derived precursors

ObjectiveTo investigate the isolation of skin-derived precursors(SKPs) from the skin of paraplegic patients, and induce it into nervous system cells.MethodsThe 2 cm2 skin samples were obtained from the incision edge in patients with thoracic vertebral fracture complicated with paraplegia or not, cut into 1-2 mm3 pieces, and then digested in trypsin for 4 hours.The epidermal layer was removed, and the cells were collected by centrifugation after blowing.The cell phenotype was detected by fluorescence histochemistry, and the cells were induced into the differentiation of the nervous system cells.ResultsThe SKPs could successfully be isolated from the skin of paraplegia patients and there was no statistical significance in the biological characteristics between paraplegics and non-paraplegics(P>0.05).Two kinds of cells from paraplegics and non-paraplegics could express the nestin, specific antigen marker, and be induced into neuron and Schwann cells.ConclusionsSKPs can be isolated from the skin of paraplegia patients, and induced into the nervous system cells.It is suggested that it has a good application prospect in autologous cell transplantation of spinal cord injury.