| Caprylic Acid Improves Lipid Metabolism,Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells |
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| 作者姓名： | ZHANG Xin Sheng ZHANG Peng LIU Ying Hua XU Qing ZHANG Yong LI Hui Zi LIU Lu LIU Yu Meng YANG Xue Yan XUE Chang Yong |
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| 作者单位： | Department of Nutrition,the First Medical Center of PLA General Hospital,Beijing 100853,China;Department of Nutrition,the First Medical Center of PLA General Hospital,Beijing 100853,China;Guizhou Crops of Chinese Armed Police Force,Guiyang 550001,Guizhou,China;Department of Nutrition,PLA Rocket Force Characteristic Medical Center,Beijing 100088,China |
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| 基金项目： | supported by the National Natural Science Fund of China[no.81703204]. |
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| 摘 要： | Objective This study aimed to investigate the effects of caprylic acid(C8:0) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells.Methods Fifty-six 6-week-old male C57 BL/6 J mice were randomly allocated to four groups fed a highfat diet(HFD) without or with 2% C8:0, palmitic acid(C16:0) or eicosapentaenoic acid(EPA). RAW246.7 cells were randomly divided into five groups: normal, lipopolysaccharide(LPS), LPS+C8:0, LPS+EPA and LPS+cAMP. The s...
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| 收稿时间： | 5 May 2021 |
Caprylic Acid Improves Lipid Metabolism,Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p- STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells |
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| ZHANG Xin Sheng,ZHANG Peng,LIU Ying Hua,XU Qing,ZHANG Yong,LI Hui Zi,LIU Lu,LIU Yu Meng,YANG Xue Yan,XUE Chang Yong.Caprylic Acid Improves Lipid Metabolism,Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells[J].Biomedical and Environmental Sciences,2022,35(2):95-106,中插1. |
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| Authors: | ZHANG Xin Sheng ZHANG Peng LIU Ying Hua XU Qing ZHANG Yong LI Hui Zi LIU Lu LIU Yu Meng YANG Xue Yan XUE Chang Yong |
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| Institution: | 1. Department of Nutrition, the First Medical Center of PLA General Hospital, Beijing 100853, China;2. Guizhou Crops of Chinese Armed Police Force, Guiyang 550001, Guizhou, China;3. Department of Nutrition, PLA Rocket Force Characteristic Medical Center, Beijing 100088, China |
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| Abstract: | Objective This study aimed to investigate the effects of caprylic acid (C8:0) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells. Methods Fifty-six 6-week-old male C57BL/6J mice were randomly allocated to four groups fed a high-fat diet (HFD) without or with 2％ C8:0, palmitic acid (C16:0) or eicosapentaenoic acid (EPA). RAW246.7 cells were randomly divided into five groups: normal, lipopolysaccharide (LPS), LPS+C8:0, LPS+EPA and LPS+cAMP. The serum lipid profiles, inflammatory biomolecules, and ABCA1 and JAK2/STAT3 mRNA and protein expression were measured. Results C8:0 decreased TC and LDL-C, and increased the HDL-C/LDL-C ratio after injection of LPS. Without LPS, it decreased TC in mice (P < 0.05). Moreover, C8:0 decreased the inflammatory response after LPS treatment in both mice and cells (P < 0.05). Mechanistic investigations in C57BL/6J mouse aortas after injection of LPS indicated that C8:0 resulted in higher ABCA1 and JAK2/STAT3 expression than that with HFD, C16:0 and EPA, and resulted in lower TNF-α, NF-κB mRNA expression than that with HFD (P < 0.05). In RAW 264.7 cells, C8:0 resulted in lower expression of pNF-κBP65 than that in the LPS group, and higher protein expression of ABCA1, p-JAK2 and p-STAT3 than that in the LPS and LPS+cAMP groups (P < 0.05). Conclusion Our studies demonstrated that C8:0 may play an important role in lipid metabolism and the inflammatory response, and the mechanism may be associated with ABCA1 and the p-JAK2/p-STAT3 signaling pathway. |
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