胆管阻塞性肝纤维化模型在新生幼犬中的尝试

目的尝试采用新生幼犬制作胆管阻塞性肝纤维化动物模型。方法对新生杂种幼犬进行胆总管双重结扎(胆管结扎组,n=22)或双重结扎并加以切断(胆管切断组,n=22),建立胆管阻塞性肝纤维化模型。观察手术后2、4、6周相关指标的变化情况。结果术后次日所有幼犬均出现尿色深黄,排便陶土样。术后2周时病理组织学观察可见汇管区纤维组织轻度增生;随着梗阻时间延长,局部纤维间隔形成,纤维间隔逐渐向小叶内伸展,局部具有特征性的胆栓形成;最终肝小叶结构破坏,假小叶形成。术后2周,胆管结扎组46%(6/13)的动物出现胆管再通现象,而胆管切断组21%(3/14)的动物出现胆管再通现象,两组比较有显著差异(P<0.001)。结论对幼犬进行单纯胆总管结扎或胆总管结扎后切断都可成功制作出胆管阻塞性肝纤维化模型,但后者成功率更高;幼犬胆管阻塞后形成肝纤维化比较快,自发逆转率低。

Establishment of Bile Duct Occlusive Fibrosis Model with Neonatal Dog

Objective To establish experimental bile duct occlusive fibrosis models in neonatal dogs. Methods(Neonatal dogs) were performed bile duct ligation(BDL group,n= 22) or bile duct ligation/scission(BDL/S group,(n= 22).)On the established models,subsequent clinical and serial pathological changes in the livers were studied.(Results)Twenty-four hours after surgery,all the animals presented progressive generalized malaise with yellow-white stools and dark urine. Fibrosis started two weeks postoperatively and resulted in architectural distortion later.The rate of bile duct recanalization in BDL/S group was lower than that in BDL group(P<(0.001)). Conclusion Experimental model of bile duct occlusive fibrosis in neonatal dog can be established by BDL or BDL/S,and the latter is more successful.