罗布麻总黄酮对大鼠脑缺血损伤的保护作用

目的研究罗布麻总黄酮对大鼠局灶性脑缺血再灌注损伤的保护作用及机制。方法雄性Wistar大鼠随机分为假手术组、模型组、罗布麻总黄酮(20、40、80mg/kg)组,灌胃给药,每天1次,连续5天,模型对照组和假手术组给予等体积生理盐水。采用HE染色观察脑组织病理形态学变化;采用测试药盒测定脑组织中丙二醛(malondialdehyde,MDA)含量、过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。结果罗布麻总黄酮各剂量组脑缺血2小时再灌注24小时脑梗死体积明显缩小,与模型组相比均有显著性差异。同时,罗布麻总黄酮各剂量组CAT、SOD、GSH-Px活性明显高于模型组组,MDA含量明显低于模型组。结论罗布麻总黄酮对局灶性脑缺血再灌注模型大鼠具有显著的保护作用。

The research on neuroprotection of total flavonoid from apocynum venetum in rat with focal cerebral ischemia reperfusion

Objective To research the neuroprotection of total flavonoid from apocynum venetum in rat with focal cerebral ischemia reperfusion and the mechanism. Methods In the experiment, rats were randomly divided into five groups： the model group, sham operation group, three total flavonoid groups （20, 40, 80 mg/kg dosage）. The rat in drug groups was given by lavage once a day for five consecutive days. Accordingly, the rat in the model group and sham operation group were treated with equal volume of normal saline. The activities of MDA, SOD and GSH-Px in brain tissue were determined and the pathomor- phological change in brain tissue was observed. Results the different doses of total flavonoid increased in histopathologic examination be observed compared with model group. At the same time, total flavonoid sig- nificandy inhibited the increase of concent of MDA and the decrease of activity of CAT, SOD and GSH-Px after focal cerebral ischemia reperfusion. Conclusion Total flavonoid from apocynum venetum can cut down the cerebral ischemia reperfusion injury in rat.