青蒿素对脑缺血/再灌注损伤大鼠白细胞介素-8表达的影响

目的探讨青蒿素对脑缺血/再灌注损伤大鼠白细胞介素-8(IL-8)表达的影响。方法将50只Wistar大鼠按随机数字表法分为假手术组、模型组、青蒿素低、中、高浓度组,每组10只。采用大脑中动脉闭塞法复制局灶性脑缺血/再灌注损伤大鼠模型。假手术组和模型组均用30 mL生理盐水灌胃,青蒿素低、中、高浓度组分别溶于生理盐水中共计30 mL,剂量为400、300、200 mg.kg-1,灌胃,每日1次,连续60 d。采用放射免疫法检测血清IL-8水平,反转录-聚合酶链反应和蛋白质免疫印迹法检测组织IL-8 mRNA和蛋白表达。结果与假手术组比较,模型组及青蒿素各浓度组血清IL-8水平和组织IL-8 mRNA、蛋白表达均明显升高,差异有统计学意义(P<0.05);与模型组比较,青蒿素各浓度组血清IL-8水平和组织IL-8 mRNA及蛋白表达均明显下降,差异有统计学意义(P<0.05),且以青蒿素高浓度组下降更为明显(P<0.05)。结论青蒿素可降低脑缺血/再灌注损伤大鼠IL-8的表达,可有效控制炎症反应进程。

Effect of artemisia annua on expression of interleukin-8 in rats with acute cerebral ischemic-reperfusion injury

Objective To investigate the effect of artemisia annua on the expression of interleukin-8(IL-8)in rats with acute cerebral ischemic-reperfusion injury(ACIRI).Methods A total of 50 Wistar rats were randomly divided into sham operation group,model group,artemisia annua high-,media-and low-dose groups,with 10 rats in each group.The model of ACIRI was reproduced by middle cerebral artery occlusion.Thirty millilitre physiological saline was administered intra-gastrically in sham operation group and model group,while 400,300,200 mg·kg-1 artemisia annua were administered intra-gastrically in artemisia annua high-,media-and low-dose groups,respectively.The intragastric administration was once a day for 60 days in all groups.The IL-8 level in serum was determinated by radioimmunoassay and the IL-8 mRNA and protein in tissues were detected by reverse transcription-polymerase chain reaction and Western blotting,respectively.Results Compared with the sham operation group,the levels of IL-8 in serum,IL-8 mRNA and protein in tissues were increased significantly in model group and artemisia annua high-,media-and low-dose groups(P<0.05).Compared with the model group,the levels of IL-8 in serum,IL-8 mRNA and protein in tissues were decreased significantly in artemisia annua high-,media-and low-dose groups(P<0.05),and the descent in high-dose group was the most obvious(P<0.05).Conclusion Artemisia annua can decrease the expression of IL-8 in rats with ACIRI and inhibit the inflammation process effectively.