适应教学中阴道毛滴虫快速染色方法探讨

目的探讨快速有效的阴道毛滴虫染色方法,以满足实验教学需求。方法将pH 6.4～6.8肝浸汤培养基转种2～3次的无菌阴道毛滴虫,在春秋季25℃和冬季10℃室温条件下,用体积分数为2%、5%、8%的Giemsa染液分别染色15、20、25、30、35、40 min,观察染色情况。结果体积分数5%的Giemsa染液在春秋季室温25℃染色20 min、冬季室温10℃染色25 min时,阴道毛滴虫外形清晰呈梨形,细胞核呈紫红色,细胞质呈浅蓝色,波动膜、鞭毛和轴柱均可以观察到。结论采用体积分数5%、pH 6.4～6.8的Giemsa染液,在室温25℃时染色20 min或室温10℃染色25 min染出的阴道毛滴虫标本比较理想,符合教学需求。

A fast Trichomonas vaginalis dyeing method for experimental teaching

Objective To explore more fast and effective Trichomonas vaginalis dyeing method for experimental teaching.Methods Taking the Trichomonas vaginalis which had already inoculation for 2-3 times in liver soup medium with pH value for 6.4-6.8,dying under 25 ℃ room temperature in the spring /autumn and 10 ℃ room temperature in the winter respectively,by a concentration of 2%,5% and 8% Giemsa dyeing solution,and the dyeing time were 15,20,25,30,35 and 40 minutes respectively.The staining state was observed.Results The appearance of Trichomonas vaginalis was cleared pear-shaped,purple cell nucleus and pale blue cytoplasm when dyeing 20 minutes in spring/autumn under 25 ℃ room temperature and 25 minutes in winter under 10 ℃ room temperature by volume ratio 5% Giemsa dyeing solution.The undulating membrane,mastigont and axon cylinder can be observed.Conclusion The dyeing result of Trichomonas vaginalis specimen is ideal with the pH value 6.4-6.8,volume ratio 5% Giemsa dyeing solution dyeing 20 minutes in spring/autumn under 25 ℃,and dyeing 25 minutes in winter under 10 ℃.The specimen meet the teaching demand.