正交试验设计优化复方刺山柑胶囊水提取工艺

目的优化复方刺山柑胶囊的最佳水提取工艺。方法以甘草酸铵提取率、没食子酸提取率、浸膏得率为综合评价指标,以提取次数A(1、2、3次)、提取时间B(1.0、1.5、2.0小时)和提取溶剂用量C(8、10、12倍药材量)为考察因素,采用单因素试验和L9(34)正交试验设计法优化复方刺山柑胶囊中甘草、金钱草、诃子和黄芪的最佳水提取工艺。结果方差分析结果显示,A因素对考察指标的综合影响具有显著性(P<0.05),B和C因素的影响均不显著(P>0.05)。优选的最佳水提取工艺条件为A3B1C1,即加入8倍药材重量的水,提取3次,每次提取1小时。结论优选的工艺合理可行。

Optimization on aqueous extraction technology of compound Caper fruit capsule by orthogonal experimental design

Objective To optimize the aqueous extraction technology of compound Caper fruit cap- sule. Method The extraction technology of Glycyrrhizae Radix et Rhizoma, Lysimachiae Herba, Chebu- lae Fructus, Astragali Radix were optimized by single factor design and orthogonal experiment design, tak- ing the extraction rates of ammonium glycyrrhetate, gallic acid, and total solids as indicators, with the ex- traction times （A： 1,2, 3 times, separately）, the extraction time （ B ： 1.0, 1.5, 2. 0 h, separately） and the amount of solvent （C： 8, 10, 12 times weight, separately） as three factors that required optimization. Result Variance analysis showed that factor A had a significant effect on the combined indicators （ P 〈 0. 05 ） , while factor B and C had no significance （P 〉 0. 05 ）. The best extraction condition was A3B1C1, that is 10 times the amount of water extracting 3 times, with each time 2 hours. Conclusion The optimum technology is reasonable and effective.