凝血酶敏感蛋白-1在液压冲击损伤体外培养大鼠海马神经细胞中的表达及意义

目的探讨凝血酶敏感蛋白-1(TSP-1)在液压冲击损伤大鼠体外培养海马神经细胞中的表达。方法建立体外原代培养神经细胞液压冲击损伤模型,采用甲苯胺蓝染色观察神经细胞损伤程度,免疫细胞化学染色和Western blot技术检测神经细胞中TSP-1的表达水平。结果液压冲击后,甲苯胺蓝染色显示,损伤组部分神经细胞胞体浓缩,细胞核固缩,呈深蓝色,多为坏死型损伤细胞。免疫细胞化学染色显示,对照组神经细胞内有一定量的TSP-1表达,损伤组神经细胞内TSP-1表达增多,阳性细胞数目增多。Western blot结果显示,对照组和损伤组光密度值分别为0.937±0.194与2.318±0.495,损伤组神经细胞TSP-1蛋白表达显著强于对照组(P<0.01)。结论体外培养大鼠海马神经细胞中TSP-1高表达可能在液压冲击损伤过程中起重要作用。

Expression and significance of thrombospondin-1 in rats' hippocampal neurons cultured in vitro by fluid percussion injury

Objective To investigate the expression of thrombospondin-1(TSP-1) in hippocampal neurons of rats cultured in vitro by fluid percussion injury.Methods The primary cultural neuron models of fluid percussion injury were established.The degree of neurons injury was observed by toluidine blue stain.The expression of TSP-1 in neurons was detected by Western blot and immunocytochemical stain.Results After fluid percussion,the results of toluidine blue stain showed that most post-injury neurons were necrotic cells,the cells body were condensed,and nuclei were pycnosis and dark blue.The results of immunocytochemical stain showed that there was some expression of TSP-1 in the neurons of control group,and the expression of TSP-1 and the positive cells increased in injury group.The results of Western blot showed that the optical density value was 0.937±0.194 in control group and 2.318±0.495 in injury group,the expression of TSP-1 in the neurons of injury group was significantly stronger than that in control group(P<0.01).Conclusion The increased expression of TSP-1 may play an important role in the development of neurons cultured in vitro by fluid percussion injury.