| 小鼠白血病病毒感染细胞的DNA提取及其转染作用的初步研究 |
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| 引用本文： | 孔宪寿,程立,瞿大庆,郑富众.小鼠白血病病毒感染细胞的DNA提取及其转染作用的初步研究[J].复旦学报(医学版),1991(2). |
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| 作者姓名： | 孔宪寿 程立 瞿大庆 郑富众 |
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| 作者单位： | 上海医科大学基础医学部病理生理学教研室，上海医科大学基础医学部病理生理学教研室，上海医科大学基础医学部病理生理学教研室，上海医科大学医学基础班 实习同学 |
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| 基金项目： | 上海市科委基金(873430704)，国家自然科学基金(3870664)，高校博士点科研基金(870106) |
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| 摘 要： | 从感染SRS白血病病毒的SRSV/3T3细胞系提取的DNA,在体外感染小鼠NIH/3T3细胞,在第3周出现转化细胞和呈集落性生长的转化灶。将转化细胞接种SW-1近交系新生乳鼠或裸鼠(BALB/c),在局部产生纤维肉瘤。实验结果提示,在SRSV/3T3细胞的DNA中可能含有转化基因片段。
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| 关 键 词： | 小鼠白血病病毒 转染 转化细胞 集落性生长 |
A PRELIMINARY STUDY ON TRANSFECTION BY DNA EXTRACTED FROM A MURINE LEUKEMIA VIRUS INFECTED CELL LINE |
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| Kong Xianshou,Cheng Li,Qu Daqing,Zheng Fuzhong.A PRELIMINARY STUDY ON TRANSFECTION BY DNA EXTRACTED FROM A MURINE LEUKEMIA VIRUS INFECTED CELL LINE[J].Fudan University Journal of Medical Sciences,1991(2). |
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| Authors: | Kong Xianshou Cheng Li Qu Daqing Zheng Fuzhong |
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| Abstract: | DNA was extracted from SRSV/3T3 cell line infected with SES murine leukemia virus, and then used to transfect NIH/3T3 cells in vitro. Transformed cells and cell colonies appeared significantly three weeks after transfection. Inoculation of the transformed cells into SW-1 inbred newborn mice and BALB/c nude mice induced fibrosarcomas at the local sites. These experimental results suggest that DNA of SESV/3T3 cells might contain a transforming gene. |
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| Keywords: | murine leukemia virus transfection transforming cell colony growth |
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