紫心甘薯总黄酮对糖尿病大鼠血糖及氧化应激的影响

 目的 研究紫心甘薯总黄酮（PSPF）的降糖及其抗氧化作用。方法 采用链脲佐菌素（STZ，65 mg·kg-1）腹腔注射诱导形成糖尿病大鼠模型，实验组大鼠分为正常对照组、糖尿病模型组、PSPF高剂量组（240 mg·kg-1），中剂量组（120 mg·kg-1），低剂量组（60 mg·kg-1），阳性对照组(二甲双胍，90 mg·kg-1)，PSPF灌胃4周，每天测量体重、饮食和饮水量，初期、中期、后期测量空腹血糖（FBG），最后取血测定糖化血清蛋白（GSP）、丙二醛（MDA）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（T-SOD）的变化并测量餐后2 h血糖（2 h BG）。结果 PSPF灌胃4周后，糖尿病大鼠“三多一少”症状明显改善，与模型组相比，高、中灌胃组均有效降低FBG、GSP、2 h BG、MDA，提高大鼠血清中的T-SOD、GSH含量（P<0.01）；低剂量灌胃组均可明显改善FBG、GSP（P<0.05）、MDA、T-SOD含量（P<0.01）；血糖与抗氧化指标统计学分析相关性显著（P<0.05）。结论 PSPF可以明显改善糖尿病大鼠的血糖异常，提高大鼠抗氧化能力。

Effect of Purple Sweet Potato Flavonids on Blood Glucose and Oxidative Stress in Diabetic Rats

OBJECTIVE To study hypoglycemic and antioxidant effects of purple sweet potato flavonoids (PSPF). METHODS Diabetes mellitus (DM) was induced in rats by intraperitoneal injection of streptozotocin (STZ， 65 mg·kg-1). All the rats were divided into six groups randomly， including normal control group， diabetic model group， metformin group(90 mg·kg-1)， PSPF high-dose (240 mg·kg-1)， medium dose (120 mg·kg-1) and low dose group (60 mg·kg-1)， weight，food and fluid intake were monitored every day ， fasting blood glucose （FBG）was measured every 2 week and at the end of the experiment， GSP， MDA， GSH， T-SOD changes were tested. Also， blood glucose 2 h after meal（2 h BG）was measured . RESULTS Compared with diabetic model group， diabetic symptoms were ameliorated after rats were fed with PSPF. The levels of blood glucose， GSP， 2 h BG， MDA were decreased and serum T-SOD， GSH were increased remarkably in the groups of PSPF 120 and 240 mg·kg-1（P<0.01）； the levels of blood glucose， serum GSP（P<0.05）， MDA， T-SOD（P<0.01） were improved in the group of PSPF 60 mg·kg-1.CONCLUSION Purple sweet potato flavonoids can decrease the blood glucose level and increase antioxidant capacity of diabetic rats.