RP-HPLC同时测定石胆草中4种主要成分的含量

 目的 运用反相高效液相色谱法（RP-HPLC）同时测定石胆草药材中一个苯乙醇苷成分(PGs)和3个黄酮碳苷成分(FC-GsⅠ、FC-GsⅡ、FC-GsⅢ)的含量。方法 研究优化了石胆草药材中主要成分的检测波长和色谱分离条件等，采用ZORBAX SB-C₁₈(4.6 mm×250 mm，5 μm)色谱柱，以乙腈-四氢呋喃（10∶1）-0.02%磷酸为流动相，梯度洗脱，检测波长为332 nm，石胆草中4个主要成分得到分离（Rs>1.5），历时45 min。结果 4种成分线性关系良好，r均大于0.999 0，线性范围分别为0.4~3.2 μg (PGs)、0.32~2.56 μg (FC-GsⅠ)、0.4~3.2 μg (FC-GsⅡ)、0.46~3.52 μg (FC-GsⅢ)，方法平均加样回收率>98.9%，相对标准偏差RSD<2.08%(n=6)。结论 此方法简便、快速、准确，适合用于石胆草药材的质量控制。

A Reversed-phase High Performance Liquid Chromatography(RP-HPLC) Procedure for Determination of Four Main Components in Boea hygrometrica(Bunge) R. Brown

OBJECTIVE To develop a reversed-phase high-performance liquid chromatography (RP-HPLC) procedure for simultaneous determination of one phenylethanoid glycosides (PGs) and three flavonoid C-glycosides (FC-GsⅠ, FC-GsⅡ and FC-GsⅢ) in Boea hygrometrica (Bunge) R. Brown. METHODS The detection wavelength and chromatographic separation condition were optimized. ZORBAX SB-C₁₈ column (4.6 mm×250 mm，5 μm) was used. The 4 main components were separated with acetonitrile tetrahydrofuran (10∶1) - 0.02% phosphate in 45 min with good resolution (Rs>1.5). The components were detected at 332 nm. RESULTS All correlation coefficients were greater than 0.999 0, and the linear ranges were 0.4-3.2 μg (PGs), 0.32-2.56 μg (FC-GsⅠ), 0.4-3.2 μg (FC-GsⅡ) and 0.46-3.52μg (FC-GsⅢ), respectively. The average recoveries were greater than 98.9%, and the relative standard deviations (RSD) were less than 2.08% (n=6). CONCLUSION The method is rapid, convenient and accurate, which can be used as the quality control method for Boea hygrometrica (Bunge) R. Brown.