多肽药物汇利心康对小鼠心肌肥大与肌球蛋白重链表达的影响

 目的 探讨Gq蛋白羧基端模拟肽盐酸GCIP-27（汇利心康，HLXK）对去甲肾上腺素诱导的小鼠心肌肥大和肌球蛋白重链表达的影响。方法 60只小鼠，随机分为对照组、模型组、氯沙坦组、HLXK（10、30、90 μg·kg-1）组，分别给予生理盐水、去甲肾上腺素1.5 mg·kg-1（ip，bid）、氯沙坦钾9 mg·kg-1（ig，qd）、HLXK（10、30、90 μg·kg-1；ip，bid）。连续给药20 d后，称量小鼠体重、心脏质量、左心室质量，计算心脏质量指数和左心室质量指数。采用免疫组化法测定肌球蛋白重链(myosin heavy chain，MHC)α、β在心肌组织中的表达情况。结果 去甲肾上腺素可明显诱导小鼠心肌重构。氯沙坦、HLXK（30、90 μg·kg-1；bid）能明显降低小鼠心脏质量指数和左心室质量指数，增加心肌组织中α-MHC的表达，降低β-MHC的表达。结论 汇利心康能够有效防止小鼠发生心肌肥大，纠正心肌肥大过程中的肌球蛋白重链表达失衡。

Effects of Polypeptide Drug Huilixinkang on Cardiac Hypertrophy and Expressions of Myosin Heavy Chain in Mice

OBJECTIVE To explore the effects of Huilixinkang (HLXK)， a mimetic polypeptide of Gq protein carboxyl terminus， on myocardial hypertrophy and expressions of myosin heavy chain (MHC) in mice. METHODS Sixty mice were randomly divided into 6 groups， ie control， model， losartan and HLXK (10， 30， 90 μg·kg-1) groups; and administrated with normal saline， noradrenaline 1.5 mg·kg-1 (ip， bid)， losartan 9 mg·kg-1 and HLXK 10， 30， 90 μg·kg-1 (ip， bid)， respectively. After 20 days′ treatment， all the mice were killed， and the cardiac hypertrophy indexes including heart weight index and left ventricular mass index were measured. The expressions of α-MHC and β-MHC in myocardium were determined using immunohistochemistry technique. RESULTS Noradrenaline could induce cardiac hypertrophy in mice significantly. Losartan 9 mg·kg-1， HLXK (30， 90 μg·kg-1) markedly decreased heart weight index and left ventricular mass index， elevated the expression of α-MHC and decreased the expression of β-MHC in myocardium. CONCLUSION HLXK can effectively prevent cardiac hypertrophy in mice， and balance the expression of myosin heavy chain in the process of myocardial hypertrophy.