| 多基因联合作用对kallistatin活性增强的研究 |
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| 引用本文： | 王启钊,刁勇,吕颖慧,李招发,王立强,许瑞安.多基因联合作用对kallistatin活性增强的研究[J].中国药学杂志,2011,46(23):1805-1810. |
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| 作者姓名： | 王启钊 刁勇 吕颖慧 李招发 王立强 许瑞安 |
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| 作者单位： | 华侨大学分子药物学研究所分子药物教育部工程研究中心; |
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| 基金项目： | 国家自然科学基金项目(30900822); 福建省重点项目(2010Y0036); 华侨大学校基金(JB-2R1133) |
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| 摘 要： | 目的 加强kallistatin(Kal)这一多功能内源性抗血管生成因子抗肿瘤活性。方法 将其他多个基因与Kal联合应用，利用脂质体将带有目的基因的质粒转染入内皮细胞和肿瘤细胞，分析多基因联合治疗对细胞特性的影响。结果 Kal对肺癌细胞A549、NCI-H446、SPC-A1都具抑制作用。Kal与Trail或vasostatin(Vas)联合，可加强对SPC-A1细胞的抑制作用，但三者联合该抑制作用并未进一步加强。Kal与angiostatin(Ang)、Vas 联合应用可显著增强对血管内皮细胞EVC304生长、迁移以及小管形成的抑制作用。结论 Kal基因可与多种类型的基因联合作用，增强对肿瘤细胞和血管内皮细胞的抑制作用，为肿瘤的多基因联合治疗提供了实验基础。
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| 关 键 词： | kallistatin 抗血管形成 肺癌 基因治疗 非癌基因成瘾 |
| 收稿时间： | 2011-11-11; |
Enhancing the Anti-Cancer Activities of Kallistatin via Multigene Co-Transfection |
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| WANG Qi-zhao,DIAO Yong,L Ying-hui,LI Zhao-fa,WANG Li-qiang,XU Rui-an.Enhancing the Anti-Cancer Activities of Kallistatin via Multigene Co-Transfection[J].Chinese Pharmaceutical Journal,2011,46(23):1805-1810. |
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| Authors: | WANG Qi-zhao DIAO Yong L Ying-hui LI Zhao-fa WANG Li-qiang XU Rui-an |
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| Institution: | WANG Qi-zhao,DIAO Yong,Lü Ying-hui,LI Zhao-fa,WANG Li-qiang,XU Rui-an*(Institute of Molecular Medicine,Huaqiao University,Molecular Medicine Engineering Research Center,Ministry of Education,Quanzhou 362021,China) |
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| Abstract: | OBJECTIVE Kallistatin(Kal) is a new endogenous anti-angiogenic factor.It also has a role in anti-inflammation and anti-oxidation.Moreover,it could directly inhibit the proliferation of cancer cells.The aim of this study is to enhance the activities of this multi-functional gene.METHODS Kal together with other genes was co-transfected into cells using lipofectamine 2000(Lipo),and the cell activities were measured via MTT,scratch,transwell and tube formation assays.RESULTS It was found that Kal could inhibit ... |
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| Keywords: | kallistatin anti-angiogenesis lung cancer gene therapy non-oncogene addiction |
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