HPLC-PAD测定刺五加中刺五加苷B与刺五加苷E的含量

 目的 研究HPLC同时测定刺五加中刺五加苷B与刺五加苷E的含量。方法 采用Agilent Zorbax SB-C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.5%磷酸（0~10 min，9∶91；10~30 min，9~20∶91~80），流速1.0 mL·min-1，检测波长220 nm，柱温25 ℃。结果 刺五加苷B、刺五加苷E分别在0.35~34.83与0.69~69.20 μg·mL-1内线性关系良好，其相关系数分别为0.999 9与1.000 0；精密度RSD均为0.4%，小于2%；重复性RSD分别为1.4%与1.0%，稳定性RSD分别为3.1%与3.4%，均小于5%；平均回收率（n= 9）分别为97.4%（RSD为5.5%）和102.7%（RSD为4.3%）。结论 本方法操作简便，结果可靠，重复性好，可作为刺五加及其制剂的质量控制方法。

Determination of Eleutheroside B and Eleutheroside E in Acanthopanax senticosus by HPLC-PAD

OBJECTIVE To develope a HPLC method for quantitative determination of eleutheroside B and eleutheroside E in Acanthopanax senticosus. METHODS The separation of eleutheroside B and eleutheroside E was performed on an Agilent Zorbax SB-C₁₈ column （4.6 mm ×250 mm，5 μm） by gradient elution with 0.5% aqueous phosphoric acid and acetonitrile as the mobile phase. The flow rate was 1.0 mL·min-1 at 25 ℃， and the detection wavelength was set at 220 nm. RESULTS The linear ranges of eleutheroside B and eleutheroside E were 0.35-34.83 and 0.69-69.20 μg·mL-1，respectively，and the correlation coefficient were 0.999 9 and 1.000 0， respectively. The variations for intra- and inter-day precision were less than 3.1% and 3.4%，and the accuracy（ n=9） for eleutheroside B and eleutheroside E were 97.4% （RSD=5.5% ） and 102.7% （RSD=4.3%），respectively. CONCLUSION This method is simple，accurate，reliable and reproducible for the quality control of Acanthopanax senticosus.