巴戟天药材寡糖化学成分的高效阴离子交换色谱-脉冲安培电化学检测器指纹图谱研究

 目的 建立巴戟天药材寡糖化学成分的高效阴离子交换色谱-脉冲安培电化学检测器（HPAEC-PAD）特征指纹图谱，为制定巴戟天药材质量标准提供参考数据。方法 利用HPAEC-PAD，色谱条件为，Hamilton RCX-10 (4.1 mm×250 mm，7 μm），100 mmol·L-1 NaOH溶液(A)-100 mmol·L-1 NaOH，500 mmol·L-1 NaAc混合溶液(B)流动相，梯度洗脱，流速为0.8 mL·min-1，柱温为30 ℃，检测电压0.1V，分析时间为40 min。结果 建立了巴戟天药材寡糖化学成分的HPAEC-PAD指纹图谱，并标定出巴戟天药材20个共有峰，其中3个峰经与对照品比对为蔗果三糖、耐斯糖(nystose)、1F-果呋喃糖基耐斯(1F-fructofuranosylnyslose)，经指纹图谱系统解决方案软件（ChromafingerTM）生成共有模式，并进行相似度和主成分分析。不同产地巴戟天药材均含有以上共有峰，但在共有峰的峰高上有差异，并可用于区别巴戟天及其常见混伪品。结论 利用该方法建立的巴戟天寡糖类化学成分的指纹图谱特征性和专属性强，且该方法快速、灵敏、可靠，可用于控制巴戟天药材的质量。

Study on HPAEC-PAD Fingerprint Analysis of Oligosaccharides from Morinda officinalis

OBJECTIVE To establish the chromatographic fingerprint of oligosaccharides by high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) method for the quality control of Morindae officinalis Radix. METHODS The HPAEC-PAD method was performed on a Hamilton RCX-10 (4.1 mm×250 mm，7 μm）by gradient elution with a mixed mobile phase of 100 mmol·L-1 NaOH water solution(A) -100 mmol·L-1 NaOH and 500 mmol·L-1 NaAc water solution (B) at a flow rate of 0.8 mL·min-1. The detection was done at 0.1 V and 30 ℃.The analysis time was 40 min. RESULTS The HPAEC- PAD fingerprint chromatogram of Morinda officinalis was built and 20 characteristic peaks were pointed out, of which 3 peaks were identified as kestose, nystose, and 1F-fructofuranosylnyslose by comparison with chemical reference substance. The common pattern of HPAEC-PAD fingerprints from the 10 batches of Morinda officinalis samples from GAP bases were obtained through ‘Chromafinger 2005’ solution software, and authentication and quality assessment were analyzed by Similarity and Principle Commonent Analysis. CONCLUSION The method is simple, rapid and specific. It can be used to identify Morindae officinalis Radix and evaluate its quality.