| 隐丹参酮对谷氨酸致大鼠神经细胞氧化应激的影响 |
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| 引用本文： | 张芳艳,陈少锐,梅峥嵘,刘培庆. 隐丹参酮对谷氨酸致大鼠神经细胞氧化应激的影响[J]. 中国药学杂志, 2011, 46(16): 1245-1247 |
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| 作者姓名： | 张芳艳 陈少锐 梅峥嵘 刘培庆 |
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| 作者单位： | 重庆市食品药品检验所;中山大学药学院;广州医学院第三附属医院; |
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| 基金项目： | 国家自然科学基金(30772576); 科技部重大新药创制临床前研究专项(2009ZX09102-152) |
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| 摘 要： | 目的 观察隐丹参酮（CT）对谷氨酸（Glu）损伤大鼠皮层神经元的保护作用。方法 体外培养大鼠皮层神经细胞，建立Glu损伤模型，采用MTT法检测细胞活力，比色法检测超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量；荧光法检测细胞内Ca2+的变化，Western blot检测caspase 3蛋白的表达。结果 CT能显著提高Glu损伤神经细胞的存活率，提高SOD的活性，降低MDA的生成，抑制细胞内［Ca2+］i的变化以及caspase 3蛋白表达，抑制神经细胞凋亡。结论 CT对培养神经细胞谷氨酸兴奋毒性具有显著的保护作用，其机制可能与CT拮抗Glu诱导的氧化应激以及抑制细胞内［Ca2+］i作用有关。
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| 关 键 词： | 隐丹参酮 谷氨酸 皮层神经元 凋亡 |
| 收稿时间： | 2011-11-11; |
Protective Effect of Cryptotanshinone on Glutamaic acid Induced Injury in Cortical Neurons |
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| ZHANG Fang-yan,,CHEN Shao-rui,MEI Zheng-rong,LIU Pei-qing. Protective Effect of Cryptotanshinone on Glutamaic acid Induced Injury in Cortical Neurons[J]. Chinese Pharmaceutical Journal, 2011, 46(16): 1245-1247 |
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| Authors: | ZHANG Fang-yan CHEN Shao-rui MEI Zheng-rong LIU Pei-qing |
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| Affiliation: | ZHANG Fang-yan1,2,CHEN Shao-rui2,MEI Zheng-rong3,LIU Pei-qing2(1.Chongqing Institute for Food and Drug Control,Chongqing 401121 China,2.Laboratory of Pharmacology and Toxicology,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 501180,China,3.The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China) |
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| Abstract: | OBJECTIVE To investigate the protective effect of cryptotanshinone on injury induced by glutamatic acid in rats.METHODS Cell viability was detected by MTT assay.Superoxide dismutase(SOD)activity and malondialdehvde(MDA)level were detected by colorimetry,The concentration of intracellular Ca2+ was detected with fluorescence probe.And the expression of caspase 3 was detected by Western blot.RESULTS Compared with glutamaic acid group,cryptotanshinone could increase cell survival rate and the SOD activity,decre... |
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| Keywords: | cryptotanshinone glutamate cortical neurons apoptosis |
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