| 黄芪糖蛋白对小鼠脾淋巴细胞的体外作用 |
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| 引用本文： | 杨向竹,赵俊云,薛慧清,许凯霞,冯前进,崔向青.黄芪糖蛋白对小鼠脾淋巴细胞的体外作用[J].中国药学杂志,2011,46(23):1811-1813. |
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| 作者姓名： | 杨向竹 赵俊云 薛慧清 许凯霞 冯前进 崔向青 |
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| 作者单位： | 北京中医药大学基础医学院;山西中医学院方剂教研室; |
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| 基金项目： | 山西省自然科学基金资助项目(2009011013-1); 北京中医药大学2011年自主课题资助项目 |
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| 摘 要： | 目的 研究黄芪糖蛋白（Huangqi glycoprotein,HQGP）对小鼠脾淋巴细胞体外增殖、细胞周期、分泌白介素2（IL-2）的影响。方法 MTT法测定不同浓度黄芪糖蛋白对ConA、LPS诱导的小鼠脾淋巴细胞增殖反应以及对混合淋巴反应（MLR）的影响；流式细胞法测定黄芪糖蛋白对细胞周期、脾细胞分泌IL-2的影响。结果 黄芪糖蛋白明显抑制ConA、LPS诱导的脾淋巴细胞的增殖，抑制混合淋巴反应；细胞周期分析表明，黄芪糖蛋白抑制T淋巴细胞增殖于G0G1期；抑制培养的脾细胞分泌IL-2。结论 黄芪糖蛋白在体外具有抑制小鼠脾淋巴细胞增殖的作用，其机制可能与抑制IL-2的分泌有关。
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| 关 键 词： | 黄芪糖蛋白 淋巴细胞增殖 细胞周期 白介素2 |
| 收稿时间： | 2011-11-11; |
Effects of Huangqi Glycoprotein on Mouse Lymphocytes in Vitro |
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| YANG Xiang-zhu,ZHAO Jun-yun,XUE Hui-qing,XU Kai-xia,FENG Qian-jin,CUI Xiang-qing.Effects of Huangqi Glycoprotein on Mouse Lymphocytes in Vitro[J].Chinese Pharmaceutical Journal,2011,46(23):1811-1813. |
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| Authors: | YANG Xiang-zhu ZHAO Jun-yun XUE Hui-qing XU Kai-xia FENG Qian-jin CUI Xiang-qing |
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| Institution: | YANG Xiang-zhu1,ZHAO Jun-yun1,XUE Hui-qing2,XU Kai-xia1,FENG Qian-jin2,CUI Xiang-qing1(1.Beijing University of Chinese Medicine,Beijing 100029,China,2.Shanxi University of Traditional Chinese Medicine,Taiyuan 030024,China) |
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| Abstract: | OBJECTIVE To study the effects of Huangqi glycoprotein on mouse lymphocytes proliferation,cell cycle and on IL-2.METHODS The mouse splenic lymphocytes were cultured in vitro.The lymphocytes proliferation activated by mitogens or MLR were measured by MTT,and IL-2 was determined in supernatants by flow cytometric analysis.The distribution of the cell cycle was analyzed with propidium ioldide(PI) staining.RESULTS HQGP could notably inhibit the proliferation of lymphocytes induced by mitogens in dose-dependent ... |
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| Keywords: | Huangqi glycoprotein lymphocytes proliferation cell cycle IL-2 |
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