早期糖尿病视网膜病变患者血清相关细胞因子的检测

目的 应用抗体芯片技术研究早期糖尿病视网膜病变（DR）患者血清中细胞因子表达谱及其水平变化，并探讨其临床意义。方法 32例2型糖尿病患者，其中16例合并轻中度非增殖性糖尿病视网膜病变（NPDR组），16例未合并视网膜病变（DM组）。应用Raybiotech人类细胞因子抗体芯片同步检测血清中42种细胞因子水平的变化。以8名健康志愿者作为正常对照组。结果 与DM组相比，NPDR组中性粒细胞活化剂（ENA-78）、生长相关基因（GRO）、调节正常T细胞表达和分泌的细胞因子（RANTES）、血管生成素、血管内皮细胞生长因子（VEGF）和血小板源性生长因子（PDGF）的水平均显著升高（P<0.05）；而IL-1α表达水平下降（P<0.05）。在芯片所包含的42种细胞因子中ENA-78的水平变化明显，NPDR组是DM组的1.88倍和正常对照组的3.6倍。结论 早期DR患者血清中部分细胞因子表达水平存在差异，提示炎症反应在DR发生和发展中起重要作用，细胞因子有可能成为DR临床早期预警的标志物。

Detection of serum cytokines in patients with early diabetic retinopathy

Objective To determine serum cytokine profiles and levels in patients with early diabetic retinopathy (DR) by using antibody array technology and analyze their clinical significances. Methods Among 32 patients with type 2 diabetes mellitus, 16 patients with mild to moderate non-proliferative DR were as DR group; and 16 patients without retinopathy, as diabetic control group. Eight healthy subjects were selected as normal control group. The profiles of 42 cytokines were detected by human cytokines antibody array (Raybiotech). Results Compared with diabetic control group, in DR group, levels of epithelial neutrophil-activating peptide-78 (ENA-78), growth related gene (GRO), regulated upon activation normal T cell expressed and secreted (RANTES), angiogenin, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) increased significantly (P<0.05); but IL-1α decreased (P<0.05). In DR group, the serum ENA-78 excretions were 1.88 folds and 3.6 folds in comparison to diabetic control and normal control groups, respectively. Conclusion The findings of significant changes in serum cytokine profiles of patients with early DR provide evidence to support the role of inflammation in the pathogenesis of DR. These cytokines may also provide biological markers to clinical prediction of early DR.