人参皂甙对大鼠慢性颈髓损伤中神经细胞凋亡的影响 |
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引用本文: | 何畔,王愉思,周益召,梁珊. 人参皂甙对大鼠慢性颈髓损伤中神经细胞凋亡的影响[J]. 医学临床研究, 2009, 26(10): 1873-1876 |
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作者姓名: | 何畔 王愉思 周益召 梁珊 |
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作者单位: | 湖南省人民医院骨科,湖南,长沙,410005 |
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摘 要: | 【目的】探讨大鼠慢性颈髓损伤后人参皂甙对神经细胞凋亡的影响。【方法】4月龄SD大鼠随机分为3组:A组(对照组),切开颈椎后方皮肤后即缝合;B组(模型组),破坏大鼠颈椎后柱稳定性建立慢性颈髓损伤模型,造模后立即腹腔注射生理盐水2μg/kg,以后每周注射一次;C组(人参皂甙干预组),建立模型后立即腹腔注射人参皂甙100mg/kg,以后每周注射一次;在此基础上另设D组(人参皂甙+IL-10干预组),建立模型后立即腹腔注射人参皂甙100mg/kg和IL-10 5μg/kg,以后每周注射一次。腹腔麻醉取C4~C6脊髓行组织学切片,苏木素伊红(HE)染色观察形态学变化,TUNEL免疫荧光法测定颈脊髓神经细胞凋亡情况,免疫组化法测定颈脊髓IL-10的表达。【结果】成功建立大鼠慢性颈髓损伤模型,光镜下C、D组较B组病理改变明显改善,3个月及5个月时,相同时间点B、C组IL-10的表达与A组比较均明显增高,C组高于B组有显著统计学差异(P〈0.01);且A、B、C各组内比较5个月的表达高于3个月时的表达,有显著统计学差异(P〈0.01)。5个月时,A组大鼠脊髓组织TUNEL阳性细胞极少,B组有大量的阳性细胞,C、D组有少量的阳性细胞,D组最少,但均多于A组。【结论】人参皂甙都能抑制慢性颈脊髓损伤中神经细胞的凋亡,促进脊髓神经功能恢复,其作用可能与增高IL-10表达相关,联合运用IL-10可显著提高其疗效。
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关 键 词: | 慢性颈脊髓损伤 神经细胞凋亡 人参皂甙 SD大鼠 颈髓损伤 TUNEL阳性细胞 注射生理盐水 IL-10 |
Effect of Ginsenoside on the Apoptosis of Nerve Cells in Chronic Cervical Cord Injury Rats |
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Affiliation: | HE Pan, WANG Yu si, ZHOU Yi-zhao,et al ( Department of Orthopaedics, Hunan Provincial People's Hospital, Changsha 410005, China ) |
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Abstract: | [Objective]To explore the effect of ginsenoside on the apoptosis of nerve cells in chronic cervical cord injury rats. [Methods]SD rats with the age of four-month old were randomly divided into 3 groups. Group A (control group) underwent only an skin incision in posterior cervical. In Group B (model group), the rest rats were operated by destroying cervical posterior column, and then intraperitoneally injected with saline 2μg/kg once a week. Group C (ginsenoside group) received intraperitoneal injection with ginsenoside 100 mg/ kg once a week. Group D (ginsenoside + IL-10 group) received intraperitoneal injection with ginsenoside 100 mg/kg and interleukin 10(IL-10) 5μg/kg once a week. Three months and five months respectively later, the spinal cords of C4 -C6 were collected, and then the pathological changes of paraffine tissue specimens were as sessed by light microscopy after stained with HE. IL- 10 expressions were tested by streptavidin peroxidase (SP) immunohistochemistry and the cell apoptosis was detected by TUNEL-based immunofluorescent technique. [Results]The rat model of chronic cervical cord injury was established successfully. Under light micros copy, the pathological improvements were observed in Group C and D, compared with Group B. Three months and five months later, the expression of IL -10 was significantly higher in Group B and C than that in Group A at the same time point. And it was significantly higher in Group C than that in Group B ( P 〈0.01). And the expression of IL-10 was significantly higher after 5 months than that after 3 months in Group A, B and C, respectively ( P 〈0.01). Five months later, TUNEL positive cells were the least in Group A and the highest in Group B. And the positive cells were few in Group C and D, ,and the least in Group D, but higher in Group C and D than those in Group A. [Conclusion]Ginsenoside can inhibit the apoptosis of nerve cells in chronic cervical cord injury and improve the recovery of spinal nerve function by increas |
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