| bFGF对培养的基底前脑细胞表达NOS和NGFR的作用 |
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| 引用本文： | 燕启江,谢瑶,龙大宏,张永红,姚志彬.bFGF对培养的基底前脑细胞表达NOS和NGFR的作用[J].解剖学研究,1998(2). |
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| 作者姓名： | 燕启江 谢瑶 龙大宏 张永红 姚志彬 |
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| 作者单位： | 中山医科大学人体解剖教研室脑研究室，中山医科大学人体解剖教研室脑研究室，中山医科大学人体解剖教研室脑研究室，中山医科大学人体解剖教研室脑研究室，中山医科大学人体解剖教研室脑研究室 广州市510089新疆石油管理局职工医院，克拉玛依市834000，广州市510089新疆石油管理局职工医院，克拉玛依市834000，广州市510089新疆石油管理局职工医院，克拉玛依市834000，广州市510089新疆石油管理局职工医院，克拉玛依市834000，广州市510089新疆石油管理局职工医院，克拉玛依市834000 |
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| 摘 要： | 目的:本实验研究碱性成纤维细胞生长因子(bFGF)对离体的基底前脑细胞表达一氧化氮合酶(NOS)和神经生长因子受体(NGFR)的影响。方法:用bFGF培养液和普通对照培养液对胚胎14天大鼠基底前脑细胞进行培养,分别取培养7、14、21天的神经细胞做NOS酶组织化学染色和NGFR免疫组织化学反应染色。结果:用bFGF培养液培养的神经细胞在14天和21天后NOS和NGFR阳性表达明显强于对照组。结论:bFGF可明显促进培养的基底前脑细胞生长并促进NOS和NGFR的表达。
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| 关 键 词： | 碱性成纤维细胞生长因子 基底前脑 细胞培养 一氧化氮合酶 神经生长因子受体 |
EFFECT OF BFGF ON THE EXPRESSION NOS AND NGFR OF NEURAL CELLS CULTURED FROM BASAL FOREBRAIN |
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| Yan Qijiang,Xie Yao,Long Dahong,Zhong Yonghong,Yao Zhibing.EFFECT OF BFGF ON THE EXPRESSION NOS AND NGFR OF NEURAL CELLS CULTURED FROM BASAL FOREBRAIN[J].Anatomy Research,1998(2). |
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| Authors: | Yan Qijiang Xie Yao Long Dahong Zhong Yonghong Yao Zhibing |
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| Abstract: | Objective: The objective of this article is to study the effect of bFGF on the expression NOS and NGFR of neural cells from basal forebrain in vitro. Method: The cells of basal forebrain in embryo 14th day were cultuered with bFGF media and control media. The cultured cells were stained with the NADPH-d method and NGFR immunocytochemistry in the 7th day, the 14th day and 21th day. Result and Conelusion: The results show that bFGF could promote the growth of cultured basal forebrain cells and increase the expression of the NOS and NGFR cells. |
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| Keywords: | Cell culture bFGF NOS NGFR Basal forebrain |
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