隔离层在组织工程骨软骨复合支架中的作用

目的研究隔离层在一种新型组织工程骨软骨复合支架中对支架生物力学的影响以及在修复关节骨软骨缺损中的作用。方法在体外，检测具有隔离层的实验组骨软骨复合支架和没有隔离层的对照组支架的最大抗拉、抗剪切强度。将向软骨方向和成骨细胞方向诱导后的兔骨髓间充质干细胞（BMSCs），分别接种在实验组和对照组的骨软骨复合支架的软骨支架和骨支架上，然后将两组骨软骨复合支架分别回植于兔自体股骨髁间窝骨软骨全层缺损处。术后3、6个月取材，对新生骨软骨组织的Micro-CT三维重建和HE组织学染色的结果进行观察；对新生软骨下骨的骨矿物质密度（BMD）、新生软骨的压缩模量进行检测。结果具有隔离层的实验组骨软骨复合支架的最大抗拉和抗剪切强度明显高于对照组（P〈0．05）；实验组新生骨软骨的Micro—CT三维重建和HE组织学染色的形态明显优于对照组；实验组新生软骨下骨的BMD值与新生软骨的压缩模量明显高于对照组（P〈0．05）。结论隔离层能显著增强骨软骨复合支架的力学性能，并显著提高骨软骨复合支架修复骨软骨缺损的能力。

The effect of insulation layer on the tissue-engineering osteochondral composite scaffold

Objective To investigate the effect of the insulation layer in a novel tissue-engineering composite osteochondral scaffold on the biomechanics of the scaffolds and repair of articular osteochondral defect. Methods The maximal anti-tensile and anti-shear strength of the insulation layer-containing and -free osteochondral composite scaffolds were detected in vitro. The bony and chondral scaffolds were seeded with osteoblast- or chondrocyte-induced BMSCs respectively. Then, the biphasic scaffolds-cells constructs were implanted into osteochondral defects of rabbits＂ autogeneic knees. The Micro-CT 3-D reconstruction image and HE staining of newborn osteochondral tissue were analysed, and the BMD of subchondral bone and compressive modulus of neocarfilage were detected at 3 and 6 months after surgery. Results The maximal anti-tensile and anti-shear strength of experimental group were significantly higher than those of control group （P 〈0.05）. The Micro-CT 3-D reconsturction image and HE staining of newborn osteochondral tissue of experimental group were much superior than those of control group. The BMD of newborn subchondral bone and compressive modulus of neocartilage of experimental group were significantly higher than those of control group （P 〈0.05）. Conclusion The insulation layer can significantly enhance the biomechanical property of osteochondral composite scaffolds and regeneration of osteochondral tissue.