| HPLC法测定左亚叶酸钙中的右旋异构体△ |
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| 引用本文： | 刘凤丽,沈园园.HPLC法测定左亚叶酸钙中的右旋异构体△[J].北方药学,2013,0(8):1-2. |
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| 作者姓名： | 刘凤丽 沈园园 |
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| 作者单位： | 上海交通大学药学院 上海 200240;上海交通大学药学院 上海 200240 |
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| 基金项目： | 上海交大医工结合基金(No.YG2011MS31)。 |
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| 摘 要： | 目的:建立测定左亚叶酸钙原料药中右旋异构体含量的高效液相色谱方法。方法:采用CHIRAL-HSA色谱柱(150mm×4.0mm,5μm),流动相为0.1mol·L-1磷酸二氢钠缓冲液(用氢氧化钠溶液调节pH至5.0)-异丙醇-乙腈(890∶95∶15);流速为1.0mL·min-1;检测波长为286nm;柱温为40℃。结果:在1.5～10.0μg·mL-1范围内线性回归方程为Y=29.125X-1.0025(r=1.0000),平均回收率为100.1%(RSD=0.8%,n=9)。结论:该方法操作简便、灵敏度高、重复性好。
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| 关 键 词： | 高效液相色谱法 左亚叶酸钙 右旋异构体 |
Determination of dextroisomer in Calcium levofolinate by HPLC |
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| Liu Fengli
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Shen Yuanyuan.Determination of dextroisomer in Calcium levofolinate by HPLC[J].BEIFANG YAOXUE,2013,0(8):1-2. |
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| Authors: | Liu Fengli Shen Yuanyuan |
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| Institution: | Liu Fengli Shen Yuanyuan(School of pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China) |
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| Abstract: | Objietive：To establish a HPLC method for determination the content of dextroisomer in Calcium levofolinate.Methods：The separation was performed on a human albumin coated silica gel column （150mm×4.0mm,5μm）.The mobile phase was 0.1mol·L-1 sodium dihydrogen Phosphate buffer（adjust to pH 5.0 with sodium hydroxide solution）-2-propanol-acetonitrile（890：95：15）.The flow rate was 1.0 mL·min-1 .Ultraviolet absorption detector was set at 286 nm and column temperature was at 40℃.Results：The linear range of dextroisomer was between 1.5 and 10.0 μg·mL-1 （r=1.0000）.The average recovery was 100.1 % with RSD was 0.8%.Conclusions：A reproducible and specific method is provided for the content of dextroisomer in Calcium levofolinate . |
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| Keywords: | HPLC Calcium levofolinate Dextroisomer |
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