树肝细胞的分离培养及其意义

本文采用体外简易两步灌流法和激素限定RPMI1640培养液对树鼯肝细胞进行分离与培养。结果显示：分离所获肝细胞总数约为1．8×108个，细胞存活率达94％，肝细胞贴壁伸展能力强，培养时间较长，2周内保持良好的形态特征。认为分离培养的树鼯肝细胞可用作肝病研究的体外细胞模型，有助于人肝炎病毒感染的进一步研究。

Insolate and Culture of Tupaia Hepatocytes and Its Significance

In this paper,Tupaia hepatocytes were insolated by a single twe-stage perfusion method and cultured in thehormonally defined PRMI 1640 medium. The results showed that the total gield amounted to 1.8×108 cells witha viability of 94%. The Tupaia hepatocytes were of a better adhesion and spread and longer survival time withpreservation of good morphologic appearance. It is considered that Tupaia hepatocytes isolated and cultured bythe above method can be used as a cell model for the study of liver disease incoluding the research of hepatitis viral inferction.