| 兔抗人ERA多克隆抗体的纯化 |
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| 引用本文： | 万亚坤,张俊杰,陈南春,陈苏民. 兔抗人ERA多克隆抗体的纯化[J]. 细胞与分子免疫学杂志, 2001, 17(2): 166-168 |
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| 作者姓名： | 万亚坤 张俊杰 陈南春 陈苏民 |
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| 作者单位： | 1. 第四军医大学生化与分子生物学教研室，陕西西安 710032;西北农林科技大学生命科学院，陕西杨凌 712100
2. 第四军医大学生化与分子生物学教研室，陕西西安 710032 |
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| 基金项目： | 国家自然科学基金资助, No.39870380; 全军医药卫生科研基金资助， No.98M108 |
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| 摘 要： | 目的纯化兔抗人ERA的多克隆抗体。方法在大肠杆菌中，表达重组MBP-hEra融合蛋白。表达产物先继以直链淀粉树脂亲和柱和Superose12凝胶柱过滤纯化。将纯化的MBP-hEra偶联于NHS-activatedSepharoseTM上，制备亲和层析柱，纯化兔抗人ERA多克隆抗体。结果①表达、纯化的MBP-hEra，相对分子质量(Mr)为78×103，其纯度为95％。②从抗血清中纯化获得可与MBP-hEra特异性结合的兔抗hEra多克隆抗体。结论获得了特异性较好的纯化兔抗hEra多克隆抗体。
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| 关 键 词： | 人ERA 多克隆抗体 纯化 era基因 |
| 文章编号： | 1007-8738(2001)02-166-03 |
Purification of rabbitanti-human ERA polyclonal antibody |
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| WAN Ya kun,,ZHANG Jun jie,CHEN Nan chun,CHEN Su min. Purification of rabbitanti-human ERA polyclonal antibody[J]. Chinese journal of cellular and molecular immunology, 2001, 17(2): 166-168 |
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| Authors: | WAN Ya kun ZHANG Jun jie CHEN Nan chun CHEN Su min |
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| Affiliation: | WAN Ya kun1,2,ZHANG Jun jie1,CHEN Nan chun1,CHEN Su min1 1Department of Biochemistry and Molecular Biology,Fourth Military Medical University,Xi'an 710033,Shaanxi Province,China 2College of Life Sciences,Northwest Sci Tech University of Agricultu |
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| Abstract: | Aim To purify rabbit anti-hEra polyclonal antibody. Methods Therecombinant fusion protein MBP-hEra was expressed in the E.coli and was purified through amylose resin chromatography column and Superose 12 gel fitration.The purified MBP-hEra was coupled to the NHS-activated sepharoseTM to prepare affinity chromatography column to purify rabbit anti-hEra polyclonal antibody. Results ① MBP-hEra was expressed and purified successfully with relative molecolar mass(Mr)of 78× 103and its purity could reach about 95％ .② purified Rabbit anti-hEra polyclonal antibody could bind specifically to MBP-hEra. Conclusion The purified rabbit anti-hEra polyclonal antibody has better specificity and purity. |
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| Keywords: | human ERA polyclonal antibody purification |
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