基于IL-3及G-CSF的玉屏风散调控变应性鼻炎肥大细胞活性的实验研究

目的探讨并阐释中医经方玉屏风散调控变应性鼻炎肥大细胞活性的作用机制。方法选用健康SD大鼠,采用卵蛋白联合Al（OH）3复制变应性鼻炎动物模型,用玉屏风散治疗。实验结束后,收集大鼠鼻腔灌洗液,采用ELSIA法检测IL-3及G-CSF含量;处死动物,剖取鼻黏膜,匀浆,取上清液,采用Western blot法检测IL-3及G-CSF的表达;另取鼻黏膜行吉姆萨染色,进行病理检测。结果模型组动物出现明显过敏症状,玉屏风散治疗后症状明显改善,过敏症状计分差异有统计学意义（P〈0.05,P〈0.01）;模型组动物鼻腔灌洗液中IL-3及G-SCF含量最低,玉屏风散治疗后明显升高,差异有统计学意义（P〈0.05,P〈0.01）;模型组动物鼻黏膜组织匀浆中IL-3及G-SCF低表达,玉屏风散治疗后表达量明显升高,差异有统计学意义（P〈0.05,P〈0.01）;模型组动物鼻黏膜中吉姆萨染色显示肥大细胞数量明显增多,玉屏风散治疗后数量明显减少。结论玉屏风散是通过激活肥大细胞生长因子IL-3、抑制肥大细胞的增值因子G-SCF,通过对肥大细胞进行双向调节而有效地治疗变应性鼻炎。

Experimental Research of Yupingfeng Powder in Regulating the Activity of Mast Cells in the Allergic Rhinitis Based on Interleukin-3 and GM-CSF

Objective To explore and explain the action and mechanism of Yupingfeng powder in regulating the activity of mast cells in the allergic rhinitis.Methods Healthy Sprague-Dawley rats were selected to establish allergic rhinitis animal model by combing the egg protein with Al（OH）3,and then the model rats were administrated with Yupingfeng powder by oral.In the end of the experiment,the nasal lavage fluid（NLF）of the rats were collected and IL-3and G-SCF contents were detected by enzyme linked immunosorbent assay（ELSIA）method.Nasal mucosa was collected and homogenized for Western blot analysis,and another nasal mucosa was pathologically detected by giemsa color staining.Results Obvious allergic symptoms occurred in animal model group and the scores of allergic symptoms were decreased remarkably after administration with Yupingfeng powder（P〈0.05,P〈0.01）;the IL-3and G-SCF contents in the NLF of animal model group were significantly decreased and then were recovered greatly after administration with Yupingfeng powder（P〈0.05,P〈0.01）;the expression of IL-3and G-SCF in the mucosa of animal model group was down-regulated evidently and then up-regulated after Yupingfeng powder（P〈0.05,P〈0.01）;and the numbers of mast cells in nasal mucosa of animal model group were massive and reduced after administration with Yupingfeng powder.Conclusion Yupingfeng powder could effectively treat the allergic rhinitis by activating IL-3,growth factor of mast cells and inhibiting GM-SCF and inhibitory factor of mast cells proliferation,which reflects the two-way regulatory theory of traditional Chinese medicine.