锰对大鼠睾丸抗氧化能力及生精细胞凋亡的影响研究

目的:研究氯化锰对SD大鼠睾丸抗氧化能力和生精细胞凋亡的影响。方法:24只健康雄性SD大鼠随机分为3组。染锰组给予MnCl2.4H2O(15mg/kg、30mg/kg)4周,对照组给予生理盐水,各组给药途径均为腹腔注射,每天1次,每周5天。应用原位末端脱氧核苷酸转移酶标记技术检测睾丸生精细胞凋亡;化学比色法检测睾丸组织超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性。结果:生精细胞凋亡指数染锰组明显高于对照组,且随染锰剂量的增加而升高(P<0.01);睾丸组织超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性均染锰组明显低于对照组(P<0.01)。结论:锰诱导的抗氧化能力下降可能是大鼠生精细胞凋亡增加的重要原因之一。

Effect of Manganese on Testis Antioxidative Capacity and Apoptosis in Spermatogenic Cell of Rats

Objective:To study the effect of manganese chloride on testis antioxidative capacity and apoptosis in spermatogenic cell of rats.Methods:24 healthy male SD rats were randomly separated into three groups.Two groups were received intraperitoneally MnCl2·4H2O(15mg/kg,30mg/kg)for 4 weeks.The third group served as control and received normal saline(NS).All rats were injected by intraperitoneal injection once everyday and 5 times in a week.The spermatogenic cell apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling technique.The activity of superoxide dismutase,catalase and glutathione peroxidase in testis were measured by colorimetric analysis.Results:Apoptosis index of contaminated manganese groups were significantly higher than that of control group and increased with dosage of manganese(P<0.01).The activity of superoxide dismutase,catalase and glutathione peroxidase in testis in contaminated manganese group were significantly lower than control group(P<0.01).Conclusion:Decreasing of antioxidase activities induced by manganese might be one of important reasons which can lead to the increasing of rat spermatogenic cell apoptosis.