凋亡酶激活因子与p53及Ki-67在结直肠癌和结直肠腺癌组织中的表达

目的探讨凋亡酶激活因子1（Apaf-1）、p53和Ki-67在结直肠腺瘤、结直肠癌组织中的表达情况。方法采用SP染色法,检测结直肠癌、腺瘤组织中Apaf-1、p53和Ki-67的表达情况。结果结直肠癌淋巴结转移组中,Apaf-1、p53、Ki-67阳性表达率分别为22.7%、31.8%和90.9%,无淋巴结转移组分别为43.6%、89.7%和61.5%,差异均有统计学意义（均P〈0.05）。结直肠癌A、B期的Apaf-1、p53、Ki-67阳性表达率分别为43.6%、89.7%和61.5%,C、D期的Apaf-1、p53、Ki-67阳性表达率分别为27.7%、31.8%和90.9%。结直肠癌组织中Apaf-1阳性率（39.0%）低于腺瘤（88.0%）,p53阳性率（77.0%）和Ki-67阳性率（68.0%）均高于腺瘤（分别为49.0%、26.0%）,差异均有统计学意义（P〈0.05）。Apaf-1阳性表达与p53、Ki-67阳性表达均呈负相关（r1=-0.358,r2=-0.361,P〈0.01）。结论 Apaf-1、p53与Ki-67表达在结直肠癌、腺瘤的发生、发展与转移过程中有一定影响,对诊断结直肠癌有重要意义。

Expression of apoptotic protease activating facter-1, p53 and Ki-67 in colon cancer and colon adennm

Objective To explore the expression of apoptotic protease activating facter 1 （Apaf- 1 ）, Ki-67 and p53 in the human colon cancer and colon adenma. Methods The expressions of Apaf-1, Ki-67 and p53 were detected by immunohistochemistry in colon cancer and colon adenma. Results Tile positive expression rates of Apaf-I , Ki-67 and P-53 were 22.7% , 31.8% and 90. 9% in lymph node metastasis group respectively, which is different from those in non-lymph node metastasis group （43.6% , 89. 7% , 61.5% respectively）. The expression rates of Apaf-1, p53, Ki-67 were 43.6% , 89. 7%, 61.5% in colon tumor stages A and B respectively were different from those （22. 7% , 31.8% , 90. 9% respectively） in colon cancer stages C and D. The positive expression rate of Apaf-1 （39.0%） in tumor tissues were less than those of adenomas （88.0%） , the positive expression rates of p53 （77.0%） and Ki-67 （68.0%） were higher than those in adenoma （49. 0%, 26. 0% respectively）, with statistical significance （P 〈 0. 05）. The positive expression of p53 and Ki-67 had inverse correlation with Apaf-1 expression （r1 = -.0. 358, r2 = -0. 361, P 〈 0. 01 ）. Conclusion Apaf-1, p53 and Ki-67 play important roles in the process of the occurrence, development and metastasis of colon cancer and colon adenma, and has an important significance to instructs the diagnosis of colorectal cancer.